IndraLab

Statements

USP38 binds Flag. 4 / 4
| 4
sparser
"In addition, A549 cells were transfected with HA-UB, Flag-vector, or Flag-USP38 for 24 h and then infected with ZIKV (1 MOI)."
| PMC
sparser
"Furthermore, we analyzed the colocalization of USP38 and ADAR by immunofluorescence and found that both heterologously coexpressed ADAR-HA and Flag-USP38, as well as endogenous ADAR and USP38, were localized in the nucleus, with a strong colocalization fluorescence intensity observed ( xref D )."
39303916
sparser
"Therefore, we analyzed the ubiquitination levels of Flag-USP38 WT and Flag-USP38 C454A/H857A by coexpressing them with His-tagged ubiquitin (His-Ub) respectively and immunoprecipitating ubiquitylated proteins with a His antibody."
39303916
sparser
"To further clarify that USP38 can indeed regulate its own ubiquitination level, we coexpressed WT USP38 (Flag-USP38 WT) and two inactive mutants (Flag-USP38 C454A or C454A/H857A) with His-Ub, respectively, followed by IP analysis using Flag antibody."
39303916