IndraLab

Statements

OTUD6B binds GST. 3 / 3
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"Bacterial‐expressed GST and GST‐OTUD6B protein bound to glutathione‐sepharose 4B beads (from GE) was incubated with pVHL expressed in HEK293T cells for overnight at 4 °C. Then the beads were washed with PBS four times, followed by western blotting."
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"To this end, we purified GST‐OTUD6B from bacteria and confirmed direct binding thereof to LIN28B (Fig  xref )."
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"Next, GST‐OTUD6B was inserted into a reaction with FLAG‐purified ubiquitylated LIN28B from 293T cells."
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