IndraLab

Statements

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"In case of cellular UCHL1 study, SH-SY5Y cells were treated with GSNO (500nM) and rotenone (1muM) for 16h."
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"To identify whether UCHL1 was S nitrosylated in vitro the purified recombinant UCHL1 (2mg/ml) was treated with GSNO (10 molar excess)."
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"UCHL1 wild type and TM were treated with different concentrations of GSNO and the reaction mixture was incubated at 37degreesC for 2h."
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"Recombinant UCHL1 (200mul, 250muM) was treated with different concentrations of GSNO for 2h and loaded onto a Superdex 200, 10/30 (GE Healthcare Life Sciences, USA) gel filtration column."
28300150