IndraLab

Statements

CALM1 binds ZAR1. 6 / 6
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sparser
"To further confirm the above in vitro interaction between ZAR1 and CaM1 in plant cells, bimolecular fluorescent complementation (BiFC) and co-immunoprecipitation (Co-IP) assay were carried out."
27014878
sparser
"This strongly indicates that CaM1 interacts with ZAR1 via the CaMBD domain in vivo ."
27014878
sparser
"The interaction between ZAR1 and CaM1 was independent of the concentration of Calcium, but the interaction was enhanced when the concentration of Calcium increased from 2 to 10 μmol/L ( xref )."
27014878
sparser
"Together, these data showed that ZAR1 interacts with CaM1 via its CaM-binding domain at the plasma membrane in planta ."
27014878
sparser
"YFP fluorescence is only detected in the plasma membrane in cells co-expressing p35S : ZAR1-cYFP and p35S : CaM1-nYFP ( xref ), indicating that the interaction between ZAR1 and CaM1 occurs at plasma membrane."
27014878
sparser
"It indicates that the interaction between ZAR1 and CaM1 is required for the asymmetric division of zygote."
27014878