IndraLab

"CALM interacts with FHL2 in vitro ."

"CALM interacts with FHL2 in vivo ."

"To learn more about the interaction between CALM and FHL2, colocalization experiments were performed."

"However, more studies will be required to understand the role of the CALM–FHL2 interaction in leukemogenesis and especially its role in erythroleukemia."

"The interaction between CALM and FHL2 was confirmed by glutathione S -transferase-pulldown assay and co-immunoprecipitation experiments."

"To confirm the interaction between CALM and FHL2 in vitro , a glutathione S -transferase (GST)-pulldown assay was performed."

"These results indicate a direct physical interaction between CALM and FHL2."

"The N-terminal domain (aa 22–138) of CALM contains the ANTH domain, which specifically binds to lipids (PtdIns(4,5)P 2 ) in the plasma membrane. xref , xref , xref , xref The three-dimensional structure of the N-terminal 280 amino acids of CALM has been solved and consists of 10 α-helices, which aggregate to form a globular protein. xref The C-terminal half of CALM, on the other hand, has most likely a poorly ordered structure that contains multiple short protein interaction motifs, functioning like a flexible ‘fishing line'. xref This composition of CALM permits the establishment of interactions during clathrin-coated pit assembly. xref Interestingly, FHL2 binds to β-integrin, xref which together with clathrin, a major binding partner of CALM, has been shown to be involved in endocytosis. xref , xref Taken together, these data suggest a role for the CALM–FHL2 interaction in the complex interaction network in endocytosis."

"The in vivo interaction between CALM and FHL2 was confirmed by co-immunoprecipitation experiments."