IndraLab

Statements


| 5

sparser
"To test this hypothesis, a strain bearing GFP-Csn5 and Flag-Csn6 was generated, and coimmunoprecipitation (co-IP) assays were conducted."

sparser
"These results show that the CSN complex bear only one copy of Csn6, either a full-length or a truncated form (S6CD); while the MPN − domain fragment of Csn6, when expressed without the S6CD and incapable of integrating into the CSN complex, can still interact with over-stoichiometric amounts of Flag-Csn6 ( xref , xref , xref )."

sparser
"Interestingly, HA-S6MPN, which did not co-IP with endogenous CSN subunits, interacted with ectopically expressed Flag-Csn6 ( xref )."

sparser
"As shown in xref , Flag-Csn6 fusion proteins could be identified in proteins copurified with GFP-Csn5, further confirming the interaction between Csn5 and Csn6."

sparser
"FlagCSN6 was previously described. [ xref ] pCMV5‐Flag‐COP1 was kindly provided by E. Bianchi."