IndraLab

Statements

USP8 binds EGFR. 29 / 32
7 | 1 7 14
reach
"In EGF stimulated cells, UBPY underwent ubiquitination and bound to EGFR."
16120644
sparser
"UBPY binds to endocytosed EGFR and deubiquitinates it on early endosomes [10] ."
17720156
biogrid
No evidence text available
16120644
sparser
"However, the level of UBPY S680A binding to EGFR was comparable to that of wild-type UBPY ( Supplementary Fig. S2 )."
17720156
sparser
"When overexpressed in HeLa cells, USP8 mutations were associated with reduced EGFR ubiquitination and degradation as compared to WT controls."
28520590
reach
"We therefore examined whether the binding of UBPY to EGFR is indirect and mediated by 14-3-3 proteins which normally exist as a dimer [18]."
17720156
sparser
"We therefore examined whether the binding of UBPY to EGFR is indirect and mediated by 14-3-3 proteins which normally exist as a dimer [18] ."
17720156
biogrid
No evidence text available
25853104
trips
"In EGF-stimulated cells, UBPY underwent ubiquitination and bound to EGFR."
16120644
sparser
"Overall, these results support and extend our previous model that Usp8 and EGFR associate via multiple molecular interactions."
23333852
biogrid
No evidence text available
23153581
sparser
"Immunoblotting of the precipitates with anti-FLAG antibody showed that wild-type UBPY and UBPY S680A bind to EGFR with similar efficiency upon EGF stimulation ( Supplementary Fig. S2 ), indicating th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17720156
reach
"By conducting an Immunoprecipitation assay, we found an interaction between endogenous USP8 and EGFR in both cell lines, confirming that EGFR is a USP8 's client protein (XREF_FIG)."
25853104
biogrid
No evidence text available
25485838
reach
"Furthermore, we demonstrate that optimal Usp8 tyrosine phosphorylation is not necessary for binding of Usp8 to EGFR."
23333852
sparser
"The percentage of EGFR-positive tumors was much lower in our cohort than in a published cohort, in which USP8 mutations were associated with higher EGFR expression [ xref ]."
29600293
sparser
"We have previously shown that UBPY binds and deubiquitinates ligand-activated EGFR at early endosomes [10] ."
17720156
sparser
"This finding extends our previous observations that Usp8 is tyrosine phosphorylated upon stimulation of the EGFR and that Usp8 is recruited to the EGFR via multiple interactions including (direct) bin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21044682
reach
"It is not clear why the data of XREF_BIBR showed an opposite effect on EGFR by USP8, but it is supported by their observations that upon EGF stimulation, USP8 directly binds EGFR."
16702236
reach
"On the other hand, 14-3-3zeta has been reported to bind ligand activated EGFR [25], raising the possibility that the interaction between UBPY and activated EGFR is mediated by the 14-3-3 dimer."
17720156
sparser
"USP8 interacts with the epidermal growth factor receptor, and regulates proliferation and differentiation."
32581833
reach
"Immunoblotting of the precipitates with anti-FLAG antibody showed that wild-type UBPY and UBPY S680A bind to EGFR with similar efficiency upon EGF stimulation, indicating that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17720156
biogrid
No evidence text available
23477725
biogrid
No evidence text available
23333852
sparser
"Because USP8 has been reported to regulate a number of cancer targets including EGFR, xref – xref we first explored whether USP8 might interact with EGFR in NSCLCs, H1299 and H1650 cells."
25853104
sparser
"By conducting an Immunoprecipitation assay, we found an interaction between endogenous USP8 and EGFR in both cell lines, confirming that EGFR is a USP8’s client protein ( xref )."
25853104
biogrid
No evidence text available
25485838
sparser
"Furthermore, mutation of S680 resulted in enhanced Usp8 activity towards EGFR, although the EGFR–Usp8 interaction itself was not affected by S680 mutation."
23333852
sparser
"USP8 interacts with many substrates such as the epidermal growth factor receptor, an essential regulator of proliferation and differentiation, and regulates endosomal trafficking by ubiquitin-mediated sorting of the endocytosed cargoes ( xref ; xref ; xref )."
30988163