IndraLab

Statements

EGFR binds USP8. 39 / 42
7 | 1 8 23
reach
"Among these, Usp8/UbpY interacts with and is phosphorylated by activated epidermal growth factor receptor (EGFR), but its precise function in EGFR-signaling remains to be established."
18346885
sparser
"The potential mechanisms for the selective activation of the USP8-EGFR axis in the two types of diseases."
41331709
sparser
"USP8 is recruited to the EGFR via multiple interactions including binding of the USP8 DUB domain to ubiquitinated EGFR and recruitment of USP8 to the EGFR on endosomal membranes via the USP8 N-terminal domain [ xref ]."
40258843
biogrid
No evidence text available
16120644
biogrid
No evidence text available
23477725
reach
"In EGF stimulated cells, UBPY underwent ubiquitination and bound to EGFR."
16120644
sparser
"Under serum starvation – a standard method to promote ciliogenesis – this EGFR–USP8 signalling is reduced, allowing cilia formation to occur ( xref )."
40776899
sparser
"Regarding why the USP8-EGFR axis shows such a significant difference in pathway activation between benign pituitary adenomas and malignant tumors, there is actually still a lack of direct mechanism verification and clear preclinical studies."
41331709
sparser
"Therefore, in cancer, the USP8-EGFR axis may work synergistically with the oncogenic mutations of the core genes of the related signaling pathways."
41331709
sparser
"Given the differences in the tumor microenvironment between pituitary adenomas and cancers, the USP8-EGFR axis is highly likely to be affected differently in various tumor microenvironments, resulting in variations in the activation of downstream pathways."
41331709
biogrid
No evidence text available
25485838
biogrid
No evidence text available
25485838
sparser
"USP8 interacts with the epidermal growth factor receptor, and regulates proliferation and differentiation."
32581833
sparser
"In hepatocellular carcinoma, the USP8-EGFR axis can activate three key signaling pathways: Raf/MEK/ERK, PI3K/AKT/mTOR, and JAK/STAT, working together to drive tumor progression [ xref ]."
41331709
sparser
"The percentage of EGFR-positive tumors was much lower in our cohort than in a published cohort, in which USP8 mutations were associated with higher EGFR expression [ xref ]."
29600293
sparser
"UBPY binds to endocytosed EGFR and deubiquitinates it on early endosomes [10] ."
17720156
sparser
"We therefore examined whether the binding of UBPY to EGFR is indirect and mediated by 14-3-3 proteins which normally exist as a dimer [18] ."
17720156
sparser
"Immunoblotting of the precipitates with anti-FLAG antibody showed that wild-type UBPY and UBPY S680A bind to EGFR with similar efficiency upon EGF stimulation ( Supplementary Fig. S2 ), indicating th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17720156
trips
"In EGF-stimulated cells, UBPY underwent ubiquitination and bound to EGFR."
16120644
sparser
"When overexpressed in HeLa cells, USP8 mutations were associated with reduced EGFR ubiquitination and degradation as compared to WT controls."
28520590
reach
"Furthermore, we demonstrate that optimal Usp8 tyrosine phosphorylation is not necessary for binding of Usp8 to EGFR."
23333852
sparser
"USP8 interacts with many substrates such as the epidermal growth factor receptor, an essential regulator of proliferation and differentiation, and regulates endosomal trafficking by ubiquitin-mediated sorting of the endocytosed cargoes ( xref ; xref ; xref )."
30988163
sparser
"We have previously shown that UBPY binds and deubiquitinates ligand-activated EGFR at early endosomes [10] ."
17720156
sparser
"However, the level of UBPY S680A binding to EGFR was comparable to that of wild-type UBPY ( Supplementary Fig. S2 )."
17720156
sparser
"Because USP8 has been reported to regulate a number of cancer targets including EGFR, xref – xref we first explored whether USP8 might interact with EGFR in NSCLCs, H1299 and H1650 cells."
25853104
reach
"We therefore examined whether the binding of UBPY to EGFR is indirect and mediated by 14-3-3 proteins which normally exist as a dimer [18]."
17720156
reach
"Immunoblotting of the precipitates with anti-FLAG antibody showed that wild-type UBPY and UBPY S680A bind to EGFR with similar efficiency upon EGF stimulation, indicating that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17720156
reach
"On the other hand, 14-3-3zeta has been reported to bind ligand activated EGFR [25], raising the possibility that the interaction between UBPY and activated EGFR is mediated by the 14-3-3 dimer."
17720156
sparser
"By conducting an Immunoprecipitation assay, we found an interaction between endogenous USP8 and EGFR in both cell lines, confirming that EGFR is a USP8’s client protein ( xref )."
25853104
reach
"It is not clear why the data of XREF_BIBR showed an opposite effect on EGFR by USP8, but it is supported by their observations that upon EGF stimulation, USP8 directly binds EGFR."
16702236
sparser
"In sharp contrast to this, in pituitary adenomas, the USP8-EGFR axis does not participate in most of the aforementioned complex oncogenic signaling pathways."
41331709
biogrid
No evidence text available
23333852
sparser
"Overall, these results support and extend our previous model that Usp8 and EGFR associate via multiple molecular interactions."
23333852
sparser
"Moreover, the USP8-EGFR axis specifically focuses on the regulation of the pro-opiomelanocortin (POMC)-ACTH axis."
41331709
biogrid
No evidence text available
25853104
biogrid
No evidence text available
23153581
sparser
"This finding extends our previous observations that Usp8 is tyrosine phosphorylated upon stimulation of the EGFR and that Usp8 is recruited to the EGFR via multiple interactions including (direct) bin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21044682
reach
"By conducting an Immunoprecipitation assay, we found an interaction between endogenous USP8 and EGFR in both cell lines, confirming that EGFR is a USP8 's client protein (XREF_FIG)."
25853104
sparser
"Furthermore, mutation of S680 resulted in enhanced Usp8 activity towards EGFR, although the EGFR–Usp8 interaction itself was not affected by S680 mutation."
23333852