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USP36 binds Flag. 16 / 16
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"We transfected cells with V5-DGCR8 together with control, Flag-tagged full-length USP36, and a panel of Flag-USP36 deletion mutants followed by co-IP using anti-Flag antibody."
36950067
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"Similarly, co-IP assays using lysates from cells transfected with V5-Drosha in the absence or presence of Flag-USP36 or its deletion mutants showed that Drosha also interacts with both the N-terminus and the C-terminus of USP36 ( xref and xref )."
36950067
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"Also, endogenous DGCR8 and Drosha were immunoprecipitated with Flag-USP36 using anti-Flag antibody ( xref )."
36950067
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"Indeed, exogenously expressed V5-USP36 was co-immunoprecipitated with Flag-DGCR8 and Flag-Drosha ( xref ) and Flag-USP36 co-immunoprecipitates with V5-DGCR8 ( xref ) and V5-Drosha ( xref ) by using anti-Flag antibody in cells."
36950067
sparser
"We transfected cells with Flag-USP36 or/and Flag-Fbw7γ and performed immunoprecipitation with anti-hUTP14a antibody."
30343112
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"Cell fractionation assays also confirmed that the majority of the endogenous Las1L and Nol9 are present in the nucleolar fraction together with Flag-USP36 ( xref )."
39356143
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"Conversely, PrimPol stability was significantly increased in HEK293T cells stably expressing Flag-USP36 ( xref )."
33237263
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"Flag-USP36 and Flag-Fbw7γ co-existed in the hUTP14a-specific immunoprecipitates regardless of MG132 treatment, demonstrating that hUTP14a forms a complex with USP36 and Fbw7γ ( Fig. 3 G)."
30343112
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"Immunofluorescence staining showed that hUTP14a co-localizes with Flag-USP36 and Flag-Fbw7γ in the nucleolus whether cells were treated with MG132 or not ( Fig. 3 H), confirming that hUTP14a interacts[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30343112
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"PrimPol was also detected in immunoprecipitates using anti-Flag agarose beads from cells expressed Flag-USP36 (Figure xref )."
33237263
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"We performed an affinity purification of USP36-associated protein complexes from 293 cells stably expressing Flag-USP36 using anti-Flag antibody (M2) agarose gels, followed by elution with Flag peptides ( xref ) and MS analysis."
36912080
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"Co-IP assays confirmed that Flag-USP36 binds to EXOSC10, the ‘cap’ subunit EXOSC3/hRrp40 and the ‘ring’ subunit EXOSC4/hRrp41 in 293 (Figure xref ) and HeLa ( xref ) cells, but not with Dis3 (Figure xref ), the catalytical subunit of the RNA exosome in the nucleoplasm, consistent with the notion that USP36 interacts with the nucleolar RNA exosome."
36912080
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"To determine whether USP36 directly deubiquitinates Snail2, we co-transfected HEK293T cells with His-Snail2, HA-ubiquitin and Flag-USP36 (WT or C131A)."
35111679
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"Importantly, hUTP14a binds and co-localizes with Flag-USP36 and Flag-Fbw7γ in the nucleolus with or without MG132 treatment, demonstrating that hUTP14a forms a stable complex with USP36 and Fbw7γ."
30343112
sparser
"To understand USP36 association with the RNA exosome in cells, we extracted the nuclear fraction of 293 cells transfected with Flag-USP36 and performed SEC."
36912080
sparser
"The polyubiquitinated cIAP1 was incubated with purified Flag-USP36 (WT or C131A), followed by immunoblot."
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