IndraLab

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MTOR phosphorylates AKT1 on S473. 23 / 24
1 3 | 9 7 3
signor
"Protein kinase B (PKB, Akt) is a Ser/Thr kinase involved in the regulation of cell survival, proliferation, and metabolism and is activated by dual phosphorylation on Thr(308) in the activation loop and Ser(473) in the hydrophobic motif. It plays a contributory role to platelet function, although little is known about its regulation. In this study, we investigated the role of the mammalian target of rapamycin complex (mTORC)-2 in Akt regulation using the recently identified small molecule ATP competitive mTOR inhibitors PP242 and Torin1."
21592956
reach
"Akt-1 is also phosphorylated at S473 by the mammalian target of Rapamycin (mTOR) complex referred to as (Rapamycin-insensitive companion of mTOR and mLST8 complex) mTORC2 [XREF_BIBR - XREF_BIBR]."
23006971
rlimsp
"(c) Up to 30 μM TCN-P does not interfere with the phosphorylation of Akt1 at S473 by immunoprecipitated mTOR."
20489726
sparser
"Two phosphorylation events are necessary for maximal activation of Akt, namely phosphorylation at Thr308 and Ser473 in Akt1 (Thr309/Ser474 in Akt2 and Thr305/Ser472 in Akt3) by phosphoinositide-dependent protein kinase 1 (PDK1) and the mechanistic target of rapamycin (mTOR) complex 2 (mTORC2), respectively [ xref , xref ]."
38291468
reach
"Akt1 is phosphorylated by PDK1 at T308, and additionally at S473 by the Pl3K related kinases, mammalian target of rapamycin complex 2 (mTORC2) or DNA-PK to achieve its full kinase activity, depending on the stimulus and the cellular context."
22272775
reach
"Upon activation, PIP3 binds to the PH domain, causing conformational changes and exposing Thr309 of AKT1 for phosphorylation of the kinase domain, followed by a secondary phosphorylation of AKT1 at the C-terminal Ser473, which is mediated by the mTOR complex 2 (mTORC2)."
38542870
rlimsp
"Phosphorylation on serine 473 (Akt-473) is catalyzed by mTor in a second complex (TORC2), and Akt-473 phosphorylates Foxo3a to inhibit apoptosis."
19703998
sparser
"AKT1 S473 is phosphorylated by the mammalian target of rapamycin (mTOR) complex 2, (mTORC2) [ xref ], a seven-subunit protein complex, including the core components mTOR and RPTOR independent companion of mTOR complex 2 (RICTOR)."
35269443
reach
"Mechanistic target of rapamycin complex 2 (mTORC2) is responsible for phosphorylating Akt1 at Ser473 in the C-terminal hydrophobic motif of Akt1 (Figure 1)."
30205513
reach
"AKT1 is phosphorylated at S473 by the mechanistic target of rapamycin complex 2 (mTORC2)."
33614606
reach
"Activated by receptor tyrosine kinases (RTK), mammalian target of rapamycin complex 2 (mTORC2) phosphorylates AKT1 in the HM domain at Ser473 [15] (Figure 1)."
36497091
sparser
"This phosphorylation is essential for AKT1 activation and precedes subsequent AKT1 phosphorylation at S473 by mechanistic target of rapamycin (mTOR) complex 2 (mTORC2), to fully activate AKT [ xref , xref , xref ]."
36671478
sparser
"Activated by receptor tyrosine kinases (RTK), mammalian target of rapamycin complex 2 (mTORC2) phosphorylates AKT1 in the HM domain at Ser473 [ xref ] ( xref )."
36497091
sparser
"The membrane-associated AKT1 is phosphorylated at a second site by mammalian target of rapamycin complex 2 (mTORC2) on Ser473."
34224831
reach
"Insulin binds to the insulin receptor (INS-R) to initiate the insulin pathway signaling cascade via insulin receptor substrate (IRS) and phosphatidylinositol-3-kinase and serine/threonine protein kinase B (PKB/AKT), which is phosphorylated at serine 473 by the mechanistic target of rapamycin complex (mTORC) 2 (mTORC2), and activates an array of downstream targets including mTORC1 to initiate protein synthesis (5–11)."
35611355
signor
"The rictor-mtor complex directly phosphorylated akt/pkb on ser473 in vitro and facilitated thr308 phosphorylation by pdk1"
15718470
signor
"Mammalian TOR complex 1 (mTORC1) and mTORC2 exert their actions by regulating other important kinases, such as S6 kinase (S6K) and Akt.Recent findings have revealed novel important roles for mTORC2 in the phosphorylation of AGC kinase family members. mTORC2 phosphorylates and activates Akt, SGK, and PKC, which regulate cell survival, cell cycle progression and anabolism"
21157483
reach
"MTOR phosphorylates AKT1 at S473, and ASZ001 mouse BCC cells treated with itraconazole, a SMO inhibitor, reduces p-S473 AKT1 expression."
33617094
reach
"It was previously demonstrated by our group (Pereira et al., 2012) that rapamycin inhibits formation of mTOR complex 1 (mTORC1) and 2 and insulin stimulated phosphorylation of p70S6K and protein kinas[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23160140
sparser
"Of note, the S473D mutant of Akt1 and Akt1 phosphorylated in Ser 473 by the rictor-mTOR complex are better targets of PDK1 than nonphosphorylated Akt1 xref ."
19844585
sparser
"Furthermore, purified PDK-1 and immuno-precipitated mTOR readily phosphorylated purified Akt1 at T308 and S473 in vitro , respectively, regardless of whether or not TCN-P was present in the reaction mixture ( xref )."
20489726
biopax:phosphositeplus
No evidence text available
rlimsp
"It is very difficult to define clearly the hierarchy between RhebL1 and AKT1 since mTOR is a downstream effector of Rheb1 or RhebL1 and mTOR phosphorylates S473 of AKT1, leading to fully active form of AKT1 [33, 49]."
28209923