IndraLab

Statements

OTUB1 activates UBE2E1. 7 / 7
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"Recently, UBE2E1 was shown to autoubiquitinate itself in vitro in the presence or absence of the E3 ligase, RNF4 (30, 31), raising the possibility that OTUB1 may stabilize UBE2E1 by preventing autoubiquitination.To test the hypothesis that OTUB1 may directly inhibit UBE2E1, we assayed the autoubiquitinating activity of UBE2E1 in the presence and absence of OTUB1 in vitro."
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"In light of our observation that the absence of OTUB1 destabilizes UBE2E1 , we tested whether decreased cellular levels of OTUB1 reduce ubiquitination of H2A Lys-119 ."
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"Taken together, we provide evidence that OTUB1 rescues UBE2E1 from degradation in vivo."
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"Depletion of OTUB1 destabilizes UBE2E1 We took advantage of the ability to generate Otub1 - / - null MEFs to search for proteins whose stability depends on the presence of OTUB1 ."
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"OTUB1 rescues UBE2E1 from proteasomal degradation."
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"To confirm that OTUB1 directly regulates the stability of UBE2E1, we generated stable cell lines expressing siRNA-resistant FLAG-OTUB1 and knocked down endogenous OTUB1 by siRNA."
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"Taken together, we provide evidence that OTUB1 in rescues UBE2E1 from degradation in vivo."
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