IndraLab

Statements

USP20 binds Flag. 13 / 13
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"Recent studies reported that the posttranslational modifications of DUBs can influence their stability and deubiquitinase activity. xref , xref , xref We then ectopic expressed Flag‐USP20 in HCC cells and performed immunoprecipitation (IP)‐based MS."
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"Next, we verified the binding between USP20 and STAT3 by co‐transfection of Flag‐USP20 and His‐STAT3 plasmids into NIH3T3 cells, with no significant binding detected in the cells transfected with Flag‐USP20 or His‐STAT3 and IgG control group. (Figure  xref )."
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"To verify whether USP20 targeted HSPA2, HepG2 cells were transfected with Flag-USP20 cDNA and USP20 short hairpin RNA."
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"Interestingly, we discovered that ATR, a key player in replication stress signaling, xref was copurified with Flag‐USP20 (Figure xref )."
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"As shown in Fig. 4 H, HA-PTEN was co-immunoprecipitated with Flag-USP20 using anti-Flag antibody only when both plasmids were transfected."
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"Overexpression of Flag‐USP20 did not increase the STAT3 protein level in NIH3T3 cells ( Figure   xref )."
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"Immunoblotting suggested that co-immunoprecipitation occurred when Flag-USP20 and Myc-HSPA2 were co-expressed in HEK-293T cells ( xref )."
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"After co‐transfection of these mutants with Flag‐USP20 plasmid in HEK293T cells, result showed that the absence of amino acids 130–320, which constitute the CCD domain, disrupted the binding between STAT3 and USP20 (Figure xref , Supporting Information)."
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"As shown in xref , Myc-HSPA2 was ubiquitinated in the presence of HA-ubiquitin, and the co-expression of Flag-USP20 reduced the ubiquitination of Myc-HSPA2."
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"A consistent trend was observed in USP20 ‐NIH/3 T3 cells transferred with Flag‐USP20 and Flag‐USP20C154A (Figures  xref and xref )."
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"We examined the predicted binding interactions using PINK1 constructs containing the intact kinase domain and NT, and CTD (PINK1 111 − 581 ), the kinase domain with CTD (PINK1 156 − 581 ), and the C-lobe of the kinase domain with CTD (PINK1 300 − 581 ) to assess their binding to Flag-USP20."
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"In addition, USP20 was found to be specifically immunoprecipitated with MCL1 using HA beads in 293T cells overexpressing USP20-Flag and MCL1-HA ( Fig. 3 c)."
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"Similarly, MCL1 was found to be specifically immunoprecipitated with USP20 using Flag M2 beads in 293T cells overexpressing USP20-Flag and MCL1-HA ( Fig. 3 d)."
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