IndraLab
Statements
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"MPP + lesion reduced cell viability in a dose‐dependent manner, with an IC 50 of 5 µM. A downregulation in the dopaminergic TH gene expression was observed along lesion time, with no significant alterations in bIII‐tubulin and DAT gene expression, nor βIII‐tubulin and TH protein levels (Figure xref , Supporting Information)."
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"When maintained in serum-free NSC proliferation media, both Cic -null and control NSCs strongly expressed Nestin, as expected, and were devoid of staining for Gfap, bIII-Tubulin (as detected by Tuj1), and committed oligodendroglial markers (Fig. xref ; Supplementary Fig. xref )."
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"Neurospheroids are obtained until 14 days differentiation and are enriched in post‐mitotic neurons (Figure xref , Supporting Information), as indicated by the increased mRNA and protein levels of major neuronal (e.g., bIII‐tubulin, synaptophysin) and dopaminergic (e.g., tyrosine hydroxylase (TH), DAT) markers, peaking at 7 days of differentiation, with concomitant decrease in early neuroepithelial progenitor markers (e.g., Nestin) (Figure xref , Supporting Information)."
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"Upon differentiation, canonical neuronal markers were upregulated [about 10-fold induction of BIII-tubulin (FDR = 0.038), 30-fold induction of parvalbumin (FDR = 0.029)] together with peripheral nervous system marker genes such as peripherin (10-fold induction; FDR = 0.009) or gamma synuclein (181-fold induction; FDR = 0.002; xref )."
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"Neurosphere cultures of NSPCs have shown that, while all three pathways contributes to NPC proliferation, the inhibition of MEK/ERK increases the expression of the astrocyte marker GFAP and of the neuronal marker bIII-tubulin, influencing NPC differentiation and inhibition of mTOR impacts NPC survival [ xref ]."
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"Using polymerase chain reaction (PCR) and immunocytochemistry, it has been shown that all differentiated LCs express genes and synthesize proteins characteristic of all neurons (microtubule-associated protein 2, bIII-tubulin, synaptotagmin 1) and specifically of DAergic neurons (tyrosine hydroxylase, aromatic L-amino acid decarboxylase, DA transporter, vesicular monoamine transporter 2)."
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"To address the typically high level of variability of postmortem analyses, we normalized individual NOVA1 fluorescence intensities in MNs to their respective bIII-tubulin fluorescence intensities (Online Resource Fig. 10f), thereby successfully reducing the inter-individual variance in Ctrls by ~50% for nuclear NOVA1 signal and ~75% for cytoplasmic NOVA1 signal (Online Resource Fig. 10g)."
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"We report that the treatment of epSPCs and human pluripotent stem cell–derived neural progenitors (hPSC-NPs) with the GSK-3 inhibitor Ro3303544 activates β-catenin signaling and increases the expression of the bIII-tubulin neuronal marker; furthermore, the differentiation of Ro3303544-treated cells prompted an increase in the number of terminally differentiated neurons."
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"We performed immunohistochemical analysis within the injured epicenter for markers of neuronal lineage (bIII-tubulin and MAP2ab for neurons, GFAP for astrocytes, and O4 for mature oligodendrocytes) at 60 days post injury and quantified the staining by ImageJ analysis (Fig. xref )."
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"We observed increased expression of the bIII-tubulin neuronal marker in Ro3303544-treated neuronal precursors in culture, while further differentiation of Ro3303544-treated cells prompted higher levels of markers of terminally differentiated neurons in comparison to vehicle-treated control cells."
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"Overall, our results suggest that the treatment of spinal cord-resident mouse ependymal stem cells (epSPCs) and human neural progenitors derived from pluripotent stem cells (hESC-NP and hiPSC-NP) with a GSK-3 inhibitor (Ro3303544) increases neurogenesis, as reflected by the increased expression of β-catenin and neuronal markers (bIII-tubulin [early] and MAP2ab [mature])."
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"Following 14 days of differentiation, the majority of cNEPs differentiated into DCX + neuroblasts (44.6 ± 5.2%) and BIII-tubulin + immature neurons (30.3 ± 5.5%), with fewer cNEPs expressing the astrocytic marker GFAP (15.9 ± 10.2%) and oligodendryocyte marker Olig2 (6.8 ± 1.7%; xref ; Mujtaba et al., xref ; Payne et al., xref )."
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"Increased MAP-2 neuronal marker protein expression upon neuronal induction (p<0.05 undifferentiated hASCs vs. 28–36 days of differentiation) and increased bIII-tubulin neuronal marker protein expression upon neuronal induction (p<0.05 undifferentiated hASCs vs. 6-28-36 days of differentiation) were found."
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"piPSCs could efficiently form EBs in suspension and differentiate into cells in the three primary germ layers, including endoderm derivatives (cells expressing AFP, Sox17, GATA4, or FoxA2; pancreatic cells [Pdx1]; and hepatic cells [Albumin]), mesoderm derivatives (cells expressing Brachyury and mature beating cardiomyocytes [CT3 and MHC; xref ]), and ectoderm derivatives (neural [Sox1, Pax6] and characteristic mature neuronal [bIII-tubulin, MAP2ab] cells) ( xref , xref , and xref )."
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"To assess the potential susceptibility of motor neuronal cells to SARS-CoV-2 infection, we differentiated iPSC from 3 healthy donor individuals (1 male and 2 females, 37–49 years of age at biopsy collection; xref ) into motor neurons (iPSC-MNs) expressing neuronal (bIII-tubulin and SMI-312) and motoneuronal (ChAT, HB9) markers ( xref )."
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"A recent characterization of extracellular MV and exosomes released by mouse NB N2a cells demonstrated that they express multiple markers of the parental cell line, among which neural cell body protein UCH-L1, αII-spectrin, astroglial markers GFAP, BIII-tubulin, synaptophysin, and exosome marker Ali. xref "