IndraLab
Statements
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"When maintained in serum-free NSC proliferation media, both Cic -null and control NSCs strongly expressed Nestin, as expected, and were devoid of staining for Gfap, bIII-Tubulin (as detected by Tuj1), and committed oligodendroglial markers (Fig. xref ; Supplementary Fig. xref )."
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"Primary neurons ( Figure 1E ) and microglia ( Figures 1F and 1G ) from neurogenic and non-neurogenic regions of neonate rat brains express bIII-tubulin, which is a microtubule element almost exclusive of neurons, and ionized calcium-binding adapter molecule-1/allograft inflammatory factor-1 (Iba-1/AIF-1) and inducible nitric oxide synthase (iNOS), which are overexpressed in classically activated microglia (M1 phenotype) that protect against nanoparticulate matter (Liu et al., 2012) ."
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"Upon differentiation, canonical neuronal markers were upregulated [about 10-fold induction of BIII-tubulin (FDR = 0.038), 30-fold induction of parvalbumin (FDR = 0.029)] together with peripheral nervous system marker genes such as peripherin (10-fold induction; FDR = 0.009) or gamma synuclein (181-fold induction; FDR = 0.002; xref )."
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"Several bacterial classes were represented as high tag numbers in multiple patient samples compared to the host gene, bIII-tubulin, which was expressed in all samples (Table 2) . aproteobacteria sequence tags were identified in all patient samples and showed the greatest similarity to members of the Sphingomonadaceae family, which were of particular interest because of the ability of several members of this family to synthesize and incorporate sphingolipids including ceramide into their outer membranes [22] ."
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"Increased MAP-2 neuronal marker protein expression upon neuronal induction (p<0.05 undifferentiated hASCs vs. 28–36 days of differentiation) and increased bIII-tubulin neuronal marker protein expression upon neuronal induction (p<0.05 undifferentiated hASCs vs. 6-28-36 days of differentiation) were found."
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"piPSCs could efficiently form EBs in suspension and differentiate into cells in the three primary germ layers, including endoderm derivatives (cells expressing AFP, Sox17, GATA4, or FoxA2; pancreatic cells [Pdx1]; and hepatic cells [Albumin]), mesoderm derivatives (cells expressing Brachyury and mature beating cardiomyocytes [CT3 and MHC; xref ]), and ectoderm derivatives (neural [Sox1, Pax6] and characteristic mature neuronal [bIII-tubulin, MAP2ab] cells) ( xref , xref , and xref )."
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"Overall, our results suggest that the treatment of spinal cord-resident mouse ependymal stem cells (epSPCs) and human neural progenitors derived from pluripotent stem cells (hESC-NP and hiPSC-NP) with a GSK-3 inhibitor (Ro3303544) increases neurogenesis, as reflected by the increased expression of β-catenin and neuronal markers (bIII-tubulin [early] and MAP2ab [mature])."
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"In addition, they showed a strong immunostaining for GFAP ( Figure 3C ) that is characteristic of hAs, the NG2 proteoglycan of mature hBVPs ( Figure 3D ), bIII-tubulin ( Figure 3E ) and MAP-2 ( Figure 3F ) of primary rat neurons, and Iba-1/AIF-1 of primary rat microglia ( Figure 3G )."
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"We report that the treatment of epSPCs and human pluripotent stem cell–derived neural progenitors (hPSC-NPs) with the GSK-3 inhibitor Ro3303544 activates β-catenin signaling and increases the expression of the bIII-tubulin neuronal marker; furthermore, the differentiation of Ro3303544-treated cells prompted an increase in the number of terminally differentiated neurons."
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"Using polymerase chain reaction (PCR) and immunocytochemistry, it has been shown that all differentiated LCs express genes and synthesize proteins characteristic of all neurons (microtubule-associated protein 2, bIII-tubulin, synaptotagmin 1) and specifically of DAergic neurons (tyrosine hydroxylase, aromatic L-amino acid decarboxylase, DA transporter, vesicular monoamine transporter 2)."
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"We observed increased expression of the bIII-tubulin neuronal marker in Ro3303544-treated neuronal precursors in culture, while further differentiation of Ro3303544-treated cells prompted higher levels of markers of terminally differentiated neurons in comparison to vehicle-treated control cells."
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"Following 14 days of differentiation, the majority of cNEPs differentiated into DCX + neuroblasts (44.6 ± 5.2%) and BIII-tubulin + immature neurons (30.3 ± 5.5%), with fewer cNEPs expressing the astrocytic marker GFAP (15.9 ± 10.2%) and oligodendryocyte marker Olig2 (6.8 ± 1.7%; xref ; Mujtaba et al., xref ; Payne et al., xref )."
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"In brain organoids, cytotoxicity can be measured by fluorescent dyes (i.e., calcein AM and ethidium homodimer) paired with microscopy or flow cytometry and specific cell subsets using subset-specific markers for neurons (bIII-tubulin and MAP2), astrocytes (GFAP), and microglia (IBA-1) (148, 194) ."
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"We performed immunohistochemical analysis within the injured epicenter for markers of neuronal lineage (bIII-tubulin and MAP2ab for neurons, GFAP for astrocytes, and O4 for mature oligodendrocytes) at 60 days post injury and quantified the staining by ImageJ analysis (Fig. xref )."