IndraLab

Statements

USP13 binds Ubiquitin. 7 / 15
| 1 6
sparser
"The GST pull-down experiment indicates that full-length USP13 can bind with Ub, but not with ISG15, SUMO1 and SUMO2 ( xref )."
22216260
sparser
"Although the experimental results showed that USP13 could bind ubiquitin, bacterial-purified recombinant USP13 still exhibited only weak deubiquitination enzyme activity [ xref , xref , xref ]."
36612196
sparser
"The ZnF domain of USP13 cannot bind with ubiquitin, so that USP13 loses its ability to be activated by free ubiquitin ( xref )."
28498477
sparser
"We firstly examined whether the ZnF domain of USP13 (USP13-ZnF) interacts with Ub by isothermal titration calorimetry (ITC) experiment ( xref )."
22216260
reach
"In addition, NMR titration data reflected that M664, F666, M739, and F741 might be the key residues responsible for the binding of USP13-UBA to Ub (Zhang et al., 2011)."
35445009
sparser
"Overall, the USP13-ZnF domain cannot bind to Ub to activate USP13, whereas USP13-UBA can bind, which may partially explain the reason why USP13 displays only weak basal deubiquitination enzyme activity: the binding sites of USP13 to Ub are less than that other USP members, thus providing weaker binding affinity and consequent cleavage activity towards ubiquitin chains; There possibly exists constitutive self-inhibition for full-length USP13 supported by the interaction of UBA with ZnF domain, which is hypothesized to be released by recruitment of other proteins or modification, such as phosphorylation."
35445009
sparser
"The ZnF domain of USP13 cannot bind with Ub."
22216260