IndraLab
Statements
sparser
"The N-terminal region of helix D contains protruding Phe160 and Lys163 residues that make up an FXXK motif in binding site III that has been shown to be essential for OSM interaction with LIFR, LIFR/gp130, and OSMR/ gp130 as measured by receptor binding and cell survival assays 28 ."
reach
"To transduce its signals in human, OSM binds gp130 with low affinity and as such has little to no biological activity unless a second receptor chain is recruited, either the leukemia inhibitor factor (LIF) receptor alpha (LIFRalpha) or the specific OSM receptor beta chain (OSMRbeta)."
sparser
"Interestingly, while gp130 functions as a β-receptor for most of the cytokines in the Il-6 family, being recruited only after binding of the cytokine to its respective α-receptor (i.e., IL-6 binds first to IL-6R or soluble IL-6R, LIF to LIFR and IL-11 to IL-11R, and the complexes are then recruited to gp130), it functions as a low-affinity α-receptor for OSM, which bind to gp130 before being recruited to OSMR or LIFR [48, [51] [52] [53] [54] ."
reach
"Rather few cell types express a cell membrane-bound IL-6R and, in most cases, cells are stimulated by so called trans-signaling in which the hexameric complex is made up by IL-6 initially binding to a soluble IL-6R.Antibodies neutralizing gp130 inhibit the bone resorptive response to IL-6/soluble IL-6R in neonatal mouse calvarial bones, but do not affect the bone resorptive response to OSM, which might be related to the fact that OSM binds to a monomeric gp130, whereas IL-6/soluble IL-6R binds to a homodimer of gp130 [58]."
sparser
"This was the case for the B-K5 antibody, which antagonized the binding of OSM to gp130 but did not interfere with the signals provided by the related cytokines triggering the proliferation of the TF1 erythroleukemia cell line or the induction of haptoglobin synthesis in the HepG2 hepatoma cell line."
sparser
"To transduce its signals in human, OSM binds gp130 with low affinity and as such has little to no biological activity unless a second receptor chain is recruited, either the leukemia inhibitor factor (LIF) receptor α (LIFR α ) or the specific OSM receptor β chain (OSMR β ) (Gearing et al. xref ; Mosley et al. xref ; Tanaka and Miyajima xref )."
reach
"OSM can form a
complex with either LIFR/gp130 or OSMR/gp130 , and therefore the effects of OSM and activation of
downstream signaling pathways such as STAT3, ERK, AKT, and Src signaling in breast
cancer cells may be mediated through either LIFR or OSMR, but previous studies have
not determined which receptor is responsible."
sparser
"In contrast, OSM binds gp130 with low affinity as described by Liu et al. [ xref ] and Gearing et al. [ xref ] and as such has little to no biological activity unless a second receptor chain is recruited, either the LIFR α [ xref ] or the specific OSMReceptor-beta chain (OSMR β ) [ xref ]."
"Stimulation of cells with the interleukin-6 family of cytokines triggers homo- or hetero-dimerization of gp130. The dimerization of gp130 leads to activation of associated cytoplasmic tyrosine kinases and subsequent modification of transcription factors. Some of these biological activities of il-6 are also often exerted by other cytokines, i.e. Il-11, lif, osm, cntf, and ct-2."
reach
"This was the case for the B-K5 antibody, which antagonized the binding of OSM to gp130 but did not interfere with the signals provided by the related cytokines triggering the proliferation of the TF1 erythroleukemia cell line or the induction of haptoglobin synthesis in the HepG2 hepatoma cell line."
sparser
"For instance, oncostatin M used during day 16 to 21 of differentiation can bind to heterodimeric cell surface receptors- gp130 and either leukemia inhibiting factor receptor (LIFR) or OSM receptor-beta (OSMR-beta) resulting in activation of JAK-STAT, MAPK, and PI3K/AKT pathways ( xref ; xref ; xref ; xref )."
reach
"In contrast, OSM binds gp130 with low affinity as described by Liu et al. [XREF_BIBR] and Gearing et al. [XREF_BIBR] and as such has little to no biological activity unless a second receptor chain is recruited, either the LIFRalpha [XREF_BIBR] or the specific OSMReceptor-beta chain (OSMRbeta) [XREF_BIBR]."
reach
"On the molecular level this usage of OSM from different species results in the stimulation of different receptor complexes : human OSM exclusively binds to the type I gp130 and LIFR system in mouse cells; murine OSM, however, exclusively activates the type II gp130 and OSMR system."