IndraLab

Statements

OTUD4 binds GST. 7 / 7
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"GST-OTUD4 protein was purified using Pierce Glutathione agarose (Thermo Scientific, Maryland, USA)."
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"The his-tagged SETDB1 and flag-tagged IRTKS were purified and then added to GST beads binding GST-tagged OTUD4, the result showed that the amount of SETDB1 pulled down by the GST-OTUD4 was obviously i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
37739210
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"Purified GST, full-length, truncated GST-IRTKS, GST-OTUD4, and GST-SETDB1 constructs bound to glutathione-Sepharose 4B beads (30 μl GE) was incubated with His-tag protein."
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"GST–OTUD4 or GST protein alone, as a control, were coupled to glutathione–Sepharose beads and incubated with biotinylated RNA or without RNA."
31138677
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"Moreover, purified GST-OTUD4 could interact with recombinant His-Snail1 in vitro , indicating a direct interaction between OTUD4 and Snail1 ( Fig. 2 D)."
38040050
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"The fluorescence intensity in the presence of biotinylated RNA was more than 10-fold higher for GST–OTUD4 compared to the GST control, indicating that OTUD4 was able to bind RNA under these conditions ( xref B)."
31138677
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"Recombinant GST-OTUD4 and pET28a-Snail1 proteins were expressed in Escherichia coli strain BL21."
38040050