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YOD1 binds ZNF24. 13 / 13
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"We then conducted reciprocal coimmunoprecipitation (co-IP) experiments, which corroborated the mass spectrometry findings, revealing an interaction of endogenous YOD1 and ZNF24 in cells (Fig. xref )."
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"In addition, an in vitro Glutathione S-transferase (GST) pull-down assay revealed that the purified protein HIS-YOD1 directly bound to GST-ZNF24 but not to the GST control (Fig. xref ), thereby verifying a direct interaction between YOD1 and ZNF24."
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"Interaction of the SCAN box domain of ZNF24 with the UBX-like domain of YOD1."
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"To gain further insight into the regions necessary for the binding of YOD1 to ZNF24, we generated truncations of YOD1 (Fig. xref ) and ZNF24 (Fig. xref )."
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"Our study demonstrated for the first time that YOD1 interacted with and stabilized ZNF24, with the UBX-like domain of YOD1 binding to the SCAN box domain of ZNF24."
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"Coimmunoprecipitation assays demonstrated that both endogenous and exogenous YOD1 interact with the N-terminal SCAN box region of ZNF24 (Fig. xref )."
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"Mechanistically, YOD1 binds directly to and stabilizes ZNF24, specifically removing the K27/29/48-linked ubiquitination of ZNF24 at the K308 site."
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"Thus, we hypothesized that YOD1 may affect ccRCC progression through the ZNF24/VEGF axis.We then conducted reciprocal coimmunoprecipitation (co-IP) experiments, which corroborated the mass spectrometry findings, revealing an interaction of endogenous YOD1 and ZNF24 in cells (Fig. 3E, F)."
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"In addition, an in vitro Glutathione S-transferase (GST) pull-down assay revealed that the purified protein HIS-YOD1 directly bound to GST-ZNF24 but not to the GST control (Fig. 3H), thereby verifying a direct interaction between YOD1 and ZNF24."
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"To gain further insight into the regions necessary for the binding of YOD1 to ZNF24, we generated truncations of YOD1 (Fig. 4A) and ZNF24 (Fig. 4F)."
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"Our study demonstrated for the first time that YOD1 interacted with and stabilized ZNF24, with the UBX-like domain of YOD1 binding to the SCAN box domain of ZNF24."
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"YOD1 interacts with ZNF24 directly."
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"Collectively, these results provide evidence that the N-terminal SCAN-box domain of ZNF24 binds to the N-terminal UBX-like domain of YOD1."
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