IndraLab

Statements

USP15 binds DDX58. 20 / 23
2 | 8 10
reach
"We hypothesized that USP15 interacts with RIG-I and the interaction is independent of the DUB activity, and the coimmunoprecipitation assay proved the credibility."
26061460
reach
"Data are means ± SD from three independent experiments.Figure 7: (a) The interaction between USP15 and RIG-I was independent of the DUB activity of USP15."
26061460
sparser
"Our result showed that the presence of GST-RIG-I-N retained USP15 C250 (Fig. 7e), indicating that the interaction of RIG-I with USP15 is due to a direct physical association."
26061460
sparser
"Mechanistically, the interaction between RIG-I and USP15 suggests the model that USP15 prevents the access of a downstream target to the RIG-I complex."
26061460
sparser
"We used a coimmunoprecipitation experiment to map the domain of USP15 that facilitates its interaction with RIG-I. As demonstrated in Fig. 7c, RIG-I interacted with USP15-WT and the C-terminal UCH domain (USP15-C250), but not with the N-terminal DUSP domain (USP15-N249)."
26061460
reach
"Mechanistically, the interaction between RIG-I and USP15 suggests the model that USP15 prevents the access of a downstream target to the RIG-I complex."
26061460
sparser
"Figure 7: (a) The interaction between USP15 and RIG-I was independent of the DUB activity of USP15."
26061460
sparser
"Together, these results demonstrate that USP15 and RIG-I form a complex through the UCH domain of USP15, and that the N-terminal CARD domain and the C-terminal domain of RIG-I both bind to USP15."
26061460
biogrid
No evidence text available
26061460
sparser
"We hypothesized that USP15 interacts with RIG-I and the interaction is independent of the DUB activity, and the coimmunoprecipitation assay proved the credibility."
26061460
reach
"In addition, compared with USP15-C269A, USP15-H862A showed stronger interaction with RIG-I, suggesting that substituting the cysteine residue with an alanine may impair the combination with RIG-I.We also examined the specificity of the association between USP15 and RIG-I."
26061460
reach
"The second possibility is that USP15 interacts with RIG-I to reduce IPS-1-RIG-I binding."
26061460
sparser
"The cell lysates were immunoprecipitated with an anti-Flag antibody and immunoblotted with an anti-HA antibody. (e) GST pulldown assay to confirm the interaction between RIG-I and USP15."
26061460
sparser
"Our result showed that the presence of GST-RIG-I-N retained USP15 C250 ( xref ), indicating that the interaction of RIG-I with USP15 is due to a direct physical association."
26061460
reach
"We also examined the specificity of the association between USP15 and RIG-I."
26061460
sparser
"We used a coimmunoprecipitation experiment to map the domain of USP15 that facilitates its interaction with RIG-I. As demonstrated in xref , RIG-I interacted with USP15-WT and the C-terminal UCH domain (USP15-C250), but not with the N-terminal DUSP domain (USP15-N249)."
26061460
sparser
"We also identified the domains of RIG-I responsible for the RIG-I–USP15 interaction."
26061460
reach
"(e) GST pulldown assay to confirm the interaction between RIG-I and USP15."
26061460
biogrid
No evidence text available
26061460
reach
"Together, these results demonstrate that USP15 and RIG-I form a complex through the UCH domain of USP15, and that the N-terminal CARD domain and the C-terminal domain of RIG-I both bind to USP15."
26061460