IndraLab

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SORT1 binds KCNK2. 17 / 17
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"Mazella et al. found that Sortilin directly binds to TREK1 in the Golgi apparatus, thereby promoting TREK1 trafficking to the plasma membrane of mouse cortical neurons."
38626370
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"Since one of the major functions of sortilin is to address numerous proteins from intracellular compartments to the plasma membrane or lysosomes ( xref ; xref ), the question was “Are sortilin and TREK-1 physically associated ?” and, if yes “ Is sortilin involved in the sorting of TREK-1 ?” In this way, we have demonstrated using cross immuno-precipitation that a protein complex existed between sortilin and TREK-1, both endogenously co-localized in same neurons ( xref )."
30670975
sparser
"From the fact that NT and PE are able to bind matured sortilin, the existence of the complex between sortilin and TREK-1 prompted us to test both peptides on the channel activity."
30670975
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"In physiological conditions (XREF_FIG), TREK-1 and NTSR3 and Sortilin would associate in the TGN vesicle, where spadin is hydrolyzed by furin."
20405001
sparser
"A previous study demonstrated the interaction between the two proteins sortilin and TREK-1, in which mice with deletions of the sortilin ( sort1 ) or TREK-1 ( kcnk2 ) genes displayed a similar phenotype of resistance to depressive-like behavior during resignation tests such as the forced swimming test (FST) and the tail suspension test (TST) [ xref ]."
36233189
sparser
"The association of TREK-1 with the sorting protein sortilin prompted us to investigate the behavior of mice deleted from the gene encoding sortilin ( Sort1 −/− )."
30127743
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"In addition, physical interaction between TREK-1 and sortilin/NTSR-3 was characterized by pull down experiments."
30291907
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"As one of the first functions identified for Sortilin/NTSR3 was to address numerous proteins from the intracellular compartments to the plasma membrane or lysosomes [ xref , xref ], it was crucial to determine whether sortilin and TREK-1 were associated, and if they were, how sortilin was involved in the sorting of TREK-1."
36233189
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"This interaction between TREK-1 and NTSR3 and Sortilin led us to examine whether NT and/or the partial NTSR3 and Sortilin propeptide (i.e. spadin) were able to act on TREK-1 channel activity."
20405001
sparser
"Interestingly, sortilin physically interacts with the two pore domain potassium channel TREK-1 and the PE as well as its synthetic analog spadin is able to block the activation of TREK-1 highlighting their role in the depression pathology."
30670975
sparser
"However, a substantial quantity of Spadin was sufficient to internalize the Sortilin‐TREK1 complex and subsequently suppress the expression of TREK1. [ xref ] Therefore, the administration of Spadin partially reproduced the effect of overexpressed Sortilin on TREK channels."
38626370
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"Furthermore, co‐immunoprecipitation assays were performed to further elucidate the association between Sortilin and TREK1/TREK2."
38626370
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"This would lead to the internalization of the TREK-1 and Sortilin complex in early endosome and subsequently to its degradation."
20405001
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"However, a substantial quantity of Spadin was sufficient to internalize the Sortilin‐TREK1 complex and subsequently suppress the expression of TREK1."
38626370
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"Mazella et al. found that Sortilin directly binds to TREK1 in the Golgi apparatus, thereby promoting TREK1 trafficking to the plasma membrane of mouse cortical neurons."
38626370
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"Spadin, a propeptide released from the extracellular N‐terminal domain of Sortilin, binds directly to the TREK1/Sortilin complex and subsequently causes TREK‐1 endocytosis from the membrane."
38626370
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"The interaction between Sortilin, TREK1, and TREK2 was investigated using a co‐precipitation assay with the Pierce Classic IP Kit (Cat."
38626370