IndraLab
Statements
sparser
"These E2 enzymes have EC 50 values in the single digit micromolar range and lower the K M of OTUB1 for its substrate to 6.6 – 14 μM. Since the concentration of UBE2D3 alone has been reported to be 1.7 μM in MEF cells xref and the effective concentration of UBE2D3 needed to stimulate the DUB activity of OTUB1 is 1.6 μM ( xref ), it is likely that OTUB1:UBE2D3 form a stimulated DUB complex in cells."
sparser
"For example, (a) Ubiquitin transfer blockade: In multiple myeloma, OTUB1 interacted with the E2 enzyme UBE2D3 to inhibit ubiquitination of the transcription factor c-Maf, stabilizing its expression and promoting tumor cell survival ( xref ). (b) Direct target protein binding: OTUB1 suppressed ubiquitination of proteins (e.g., HIF-1α and RACK1) through non-catalytic binding, thereby driving tumor progression ( xref , xref ). (c) Phosphorylation-dependent functional switching: Phosphorylation of the Tyr26 residue in OTUB1 enabled its interaction with the cell cycle regulator p27, modulating p27 stability and cell cycle progression ( xref )."