IndraLab

Statements

USP10 activates Integrins. 6 / 6
| 6
reach
"USP10 overexpression increased alpha5beta1 (1.9-fold; P < 0.001) and alphav (1.7-fold; P < 0.05) integrin recycling, with a concomitant increase in biotinylated FN internalization (2.1-fold; P < 0.05) and recycling over 4 days (1.7-2.2-fold; P < 0.05)."
34665194
reach
"This resulted from post-translational removal of ubiquitin from integrins.13 Although it is assumed that reduced degradation in response to less ubiquitination and accumulation on the cell surface indicates that USP10 overexpression promotes integrin recycling instead of degradation, to prove this we subjected HCFs to a live cell confocal integrin recycling assay.HCFs were transfected with either control or USP10 cDNA and incubated for 48 hours."
34665194
reach
"We found that USP10 overexpression increased α5β1 integrin recycling by 1.9-fold (P < 0.001), and αv integrin recycling by 1.7-fold (P < 0.01) (Figs."
34665194
reach
"13 These results were bolstered by our recent studies demonstrating that knockdown of USP10 after wounding, ex vivo in porcine cornea and in vivo in rabbit corneas, significantly reduced scarring.13 21 22Because integrins are endocytosed with matrix, here we extended our previous studies by testing if reduced intracellular degradation of integrins as a result of USP10 overexpression (USP10 removes ubiquitin, integrins accumulate) would also result in USP10-driven matrix accumulation if integrin/ECM were recycled back to the cell surface together."
34665194
reach
"Furthermore, FN-EDA secretion was also significantly increased, as previously reported to result from elevated USP10-induced integrin-mediated TGFβ activity."
34665194
reach
"In terms of FN recycling, our recent work demonstrates that USP10 overexpression leads to integrin-mediated accumulation of extracellular FN (Phillips et al., 2021)."
37562219