IndraLab

Statements

Syntaxin binds KCNB1. 36 / 36
| 1 35
sparser
"Unlike the well-studied interaction between GluN2B and PSD-95, the Kv2.1–syntaxin interaction was not molecularly localized on the target protein."
| PMC
sparser
"The Kv2.1–syntaxin interaction appears to not be necessary for the basal trafficking of the Kv2.1 channel."
| PMC
sparser
"This is the first in vivo evidence that targeting the Kv2.1–syntaxin interaction can be neuroprotective, as it has been shown many times previously in in vitro models."
| PMC
sparser
"Others have sought to identify agents targeting the surge mechanism, which relies on the interaction between the C-terminus of KCNB1 and syntaxin."
35942524
sparser
"Here, we show that Kv2.1-induced facilitation of release is not restricted to neuroendocrine cells, but also occurs in the somatic-vesicle release from dorsal-root-ganglion neurons and is mediated by direct association of Kv2.1 with syntaxin."
20484665
sparser
"We showed previously that the observed increase in K(+) currents is a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-mediated process, and that the SNARE protein syntaxin binds directly to Kv2.1 channels."
24928958
sparser
"In the present study, we tested whether disrupting the interaction of Kv2.1 and syntaxin promoted the survival of cortical neurons following injury."
24928958
sparser
"These results establish that binding of syntaxin to Kv2.1 is crucial for the manifestation of oxidant-induced apoptosis, and thereby reveal a potential new direction for therapeutic intervention in the treatment of neurodegenerative disorders."
24928958
sparser
"Importantly, the physical interaction of Kv2.1 with syntaxin was shown to be involved in the facilitation of secretion from PC12 cells, which was independent of potassium currents."
18636750
sparser
"Thus, direct association of Kv2.1 with syntaxin promotes exocytosis."
17301173
sparser
"Similarly the voltage sensitive potassium channel Kv2.1 can also interact with syntaxin 1 ( xref , xref )."
19251047
sparser
"We show that impairment of the interaction of syntaxin with endogenous Kv2.1 attenuates NE release."
18167541
sparser
"The interaction of Kv2.1 with syntaxin and its insertion in the plasma membrane upon oxidative stress signals also requires the action of CamKII."
33381507
sparser
"Interestingly, targeting the Kv2.1-syntaxin interaction with a peptide mimicking the syntaxin-1A binding domain of Kv2.1 ameliorated cell death in an in vivo model of ischemic stroke ( xref )."
33381507
sparser
"Here a rational approach was applied to define the key molecular interactions between syntaxin and Kv2.1, some of which are shared with mammalian uncoordinated-18 (munc18)."
31308225
sparser
"Firstly, in view of the proposal that syntaxin lines the fusion pore xref , the Kv2.1-syntaxin interaction may promote full collapse fusion, which induces complete release, in place of the incomplete release associated with kiss and run exocytosis."
18167541
sparser
"Secondly, in view of the demonstration that Kv2.1 preferentially binds the open conformation of syntaxin xref , the Kv2.1-syntaxin interaction may influence the equilibrium between the closed and open conformations of syntaxin."
18167541
sparser
"Thirdly, the Kv2.1-syntaxin interaction may influence the equilibrium between the reserve and the releasable pools of vesicles."
18167541
sparser
"In any of these or other possible mechanisms, both our studies together provide strong evidence that the Kv2.1-syntaxin interaction exerts its effect on secretion after ATP-dependent priming of the vesicles."
18167541
sparser
"Given the proposed role for surface VAMP2 in the regulation of the vesicle cycle and the important role for the sustained Kv2.1 current in the regulation of dendritic calcium entry during high-frequency stimulation, the interaction of VAMP2 with Kv2.1 N terminus may contribute, alongside with the interaction of syntaxin with Kv2.1 C terminus, to the activity dependence of DCV release."
18542995
sparser
"Previous preclinical studies from our laboratory have shown efficacious neuroprotection in vivo by targeting the Kv2.1-syntaxin interaction (fig."
| PMC
sparser
"Strong evidence from Ilana Lotan’s lab has suggested that, in a manner independent of K + flow, Kv2.1 directly interacts with syntaxin in facilitating exocytosis, further substantiating the presence of non-canonical functions of Kv channels ( xref )."
30700930
reach
"This effort has led to the identification of a small molecule inhibitor (cpd5) of the protein–protein interaction between syntaxin and KCNB1, that has shown some efficacy in ameliorating neuronal loss in middle cerebral artery occlusion mouse model of ischemic stroke [121]."
35942524
sparser
"Disrupting the Kv2.1–Syntaxin Interaction."
| PMC
sparser
"These channel modification events increase the interaction between Kv2.1 and syntaxin that is seemingly solely necessary for apoptotic trafficking of the channel [ xref ]."
| PMC
sparser
"In a bid to further understand the Kv2.1–syntaxin interaction and to extend the effectiveness of our approach, we utilized molecular dynamic simulations to dock C1aB onto syntaxin [ xref ]."
| PMC
sparser
"We localized the Kv2.1–syntaxin interaction to a highly coordinated binding pocket centered on the syntaxin Ha helix."
| PMC
sparser
"This effort has led to the identification of a small molecule inhibitor (cpd5) of the protein–protein interaction between syntaxin and KCNB1, that has shown some efficacy in ameliorating neuronal loss in middle cerebral artery occlusion mouse model of ischemic stroke [ xref ]."
35942524
sparser
"Thus, we postulated that direct association of overexpressed Kv2.1 with syntaxin promotes exocytosis."
18167541
sparser
"Moreover, we show that impairment of the syntaxin-Kv2.1 interaction during the triggering phase is responsible for the reduced release."
18167541
sparser
"Here, we further characterized the binding of native syntaxin from PC12 cells to the Kv2.1 peptides."
18167541
sparser
"Next, in an effort to evaluate the involvement of the interaction between endogenous Kv2.1 and syntaxin in the Ca 2+ -regulated secretion from PC12 cells, we aimed to disrupt this interaction and to assay the impact on secretion."
18167541
sparser
"The above Kv2.1-derived syntaxin-binding peptides were shown to compete for the in situ association of syntaxin with Kv2.1 in PC12 cells xref and, hence, were suited for this purpose."
18167541
sparser
"This study is a direct continuation of and complement to our previous study xref ; both studies strongly suggest that Kv2.1-syntaxin interaction causes an enhancement of the release of DCVs."
18167541
sparser
"Notably, in both studies, the impact of the Kv2.1-syntaxin interaction on secretion was studied under conditions in which K + ion efflux and membrane potential changes were either minimal (secretion from living cells elicited by increasing the extracellular K + concentration ; xref or irrelevant (secretion from cells with permeable plasma membranes; this study)."
18167541
sparser
"Thus, the impact of Kv2.1-syntaxin interaction does not result from a change in the voltage-dependent gating of the channel, but rather reflects the effect of a direct interaction between the channel and the secretory machinery"
18167541