IndraLab

Statements


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"In addition, cell lysates obtained without the overexpression of HA-IK and Flag-USP47 were immunoprecipitated with the anti-USP47 monoclonal antibody."

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"Next, to examine whether USP47 directly binds to IK, cell lysates expressing HA-IK and Flag-USP47 were used in a pull-down assay with anti-HA or -Flag antibodies."

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"Moreover, ectopically overexpressed Flag-USP47 interacted with endogenous RPS2 ( xref )."

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"In contrast, the overexpression of Flag-USP47 decreased the p53 level, and it was also rescued by treatment with MG132 ( xref b)."

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"To verify the interaction of USP47 and RPS2, we performed immunoprecipitation with anti-Flag agarose after overexpressing HA-RPS2 and Flag-USP47."