
IndraLab
Statements
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"The PBMCs stored in liquid nitrogen was thawed in 37ºC before being stained with a mixture of fluorescence-labeled monoclonal antibodies for various surface makers (IgD-FITC, CD19-ECD, CD27-PC7, CD38-APCA750, IgM-PB, and CD45-KO; Beckman Coulter) to distinguish plasmablasts (IgD -IgM -CD27 + CD38 high ) and memory (IgD -IgM -CD27 + CD38 low ) B cell subpopulations."