IndraLab

Statements

GST binds COPS6. 4 / 4
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"We then mixed the same amount of each of the expressed proteins with purified GST or GST-CSN6, followed by a GST pull-down assay ( Fig. 2 B)."
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"For GST pull-down, purified GST, full-length or truncated CSN6-GST fusion proteins were mixed with His-tagged IN proteins individually, and then incubated with Glutathione-Sepharose beads for 0.5 h."
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"We then examined the effect of increasing concentrations of the purified recombinant GST-CSN6 on IN activity with GST protein as the control and found that GST-CSN6 inhibited IN activity in a dose-dep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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sparser
"Purified GST-CSN6 was relatively stable as shown in Coomassie blue staining ( Fig. 2 C)."
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