IndraLab

Statements

DTX3L ubiquitinates USP28. 5 / 5
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"DTX3L ubiquitinates USP28."
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"Similarly, when we performed ubiquitination assays for USP28 in cells that either overexpressed wild type USP28 or the inactive USP28 C171A along with DTX3L, we found that DTX3L promoted ubiquitination of USP28."
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"To further investigate whether the ubiquitination of USP28 by DTX3L renders it susceptible for proteasomal degradation, we performed an additional ubiquitination assay in the presence of either wild type ubiquitin (Ub) or three specific ubiquitin mutants."
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"Our results reveal a sophisticated feedback mechanism in which DTX3L ubiquitinates USP28, leading to its proteasomal degradation, while USP28 counteracts by deubiquitinating both itself and DTX3L. This cross-regulation fine-tunes DSB repair in multiple pathways, including non-homologous end-joining (NHEJ), homologous recombination (HR), single-strand annealing (SSA), and microhomology-mediated end joining (MMEJ)."
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"As expected, use of this mutant resulted in a loss of USP28 ubiquitination by DTX3L ( xref F)."
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