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YOD1 binds CDK1. 21 / 21
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"The result showed that FLAG was detectable when HA precipitated, and HA was also detectable when FLAG precipitated, demonstrating a direct interaction between YOD1 and CDK1(Fig.  xref G)."
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"Meantime, recombinant GST-YOD1 and HIS-CDK1 proteins were purified, and the GST pulldown results further excluded the direct interaction between YOD1 and CDK1 ( Fig. xref )."
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"Next, we expressed full-length FLAG-YOD1 and different truncation mutants in HEK293 cells and co-immunoprecipitations using the anti-FLAG antibodies or anti-CDK1 antibodies confirmed that CDK1 bound to the Znf domain of YOD1, but not to the N-terminal UBX region and central otubain region of YOD1 (Fig.  xref C)."
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"Congruent with the above results, CDK1 specifically bound to the C-terminal Znf domain of YOD1 rather than the N-terminal UBX region and central otubain region of YOD1 (Fig.  xref D)."
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"Specifically, YOD1 binds directly to CDK1, leading to de-polyubiquitylation and upregulation of CDK1 expression [ xref ]."
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"These findings indicate that CDK1 bound to the Znf domain of YOD1 and then YOD1-T-OTUD was the Ub-specific domain of YOD1 to deubiquitinate CDK1 while the N-terminal domain had no deubiquitinating effect."
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"In addition, CDK1 inhibitors could eliminate the effect of YOD1 overexpression (the proliferation and resistance to cisplatin), which further elucidates the tumor promoting mechanism of the YOD1-CDK1 axis."
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"The UBXL domain of YOD1 is essential for binding TRAF6 and p97, and YOD1 interacts with CDK1 via the C-terminal ZnF domain (Ernst et al, xref ; Han et al, xref ; Schimmack et al, xref )."
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"For example, the CDK1 inhibitor can eliminate the effects of YOD1 overexpression (proliferation and resistance to cisplatin), thereby elucidating the tumor-promoting mechanism of the YOD1-CDK1 axis [ xref ]."
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"Specifically, YOD1 binds directly to CDK1, leading to de-polyubiquitylation and upregulation of CDK1 expression [37]."
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"CDK1 binds to the ZNF domain of YOD1 and participates in the occurrence and development of triple-negative breast cancer (TNBC) [ xref ]."
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"The result showed that FLAG was detectable when HA precipitated, and HA was also detectable when FLAG precipitated, demonstrating a direct interaction between YOD1 and CDK1(Fig. 4G)."
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"We further co-expressed HA-tagged CDK1 with various Flag-tagged YOD1 mutants in HEK293 cells to identify which domain YOD1 bound to the CDK1 by performing reciprocal co-immunoprecipitation."
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"YOD1 interacts directly with CDK1."
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"Furthermore, FLAG-tagged YOD1 or HA‐tagged CDK1 was co‐transfected into 293T cells to detect interactions of exogenous YOD1 and CDK1 by immunoprecipitation."
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