IndraLab

Statements

SNHG1 decreases the amount of USP8. 5 / 5
| 5
reach
"As shown in Fig. 5A, overexpression of SNHG1 specifically inhibited USP8 expression with no remarkable effect on the expression of other potential E3 ubiquitin ligases or Ubiquitin specific peptidases in UROtsa cells."
38365726
reach
"To elucidate the mechanisms underlying SNHG1 inhibition of USP8 expression, we first evaluated the potential effects of SNHG1 on the USP8 mRNA level and USP8 promoter-driven luciferase reporter activity."
38365726
reach
"Interesting, while SNHG1 inhibited USP8 mRNA expression, it did not impact USP8 promoter-driven luciferase reporter activity (Fig. 7B), thereby ruling out transcriptional regulation of USP8 by SNHG1."
38365726
reach
"The results showed that ectopic expression of SNHG1 dramatically attenuated the USP8 mRNA level that was precipitated by the anti-HUR antibody in comparison to its scramble vector transfectant (Fig. 8D), suggesting that negative correlation of SNHG1 expression and USP8 mRNA expression.In our quest to conclusively demonstrate that SNHG1 influence over downstream genes and cellular behaviors hinges on HUR, we had successfully engineered shHUR into U5637(Vector) and U5637(SNHG1) for the targeted knockdown of HUR, as illustrated in Fig. 8E."
38365726
reach
"Additionally, we observed that overexpression of SNHG1 effectively suppressed USP8 levels, while its knockdown led to a substantial increase in USP8 expression."
38365726