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OTUD3 binds GST. 8 / 8
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"In order to observe DNA response to the reaction, less GST-OTUD3 and less reaction time were applied compared with experiments in Figure 4 F (details in STAR Methods )."
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"FLAG-cGAS bearing polyUb was purified from 293T shOTUD3#1 cells by denaturing IP and then treated with GST-OTUD3 in the absence or presence of HT-DNA in vitro ."
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"Furthermore, in vitro pull-down assays with purified recombinant proteins showed that YY1 directly bound GST-OTUD3, not GST control (Fig. xref )."
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"The results show that GST-OTUD3 removes WT polyUb on cGAS noticeably, and has specific deubiquitination activity toward K11-, K27-, and K48-linked polyUb on cGAS ( Figure 4 F)."
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"24 To see whether OTUD3 could remove this specific modification, we generated cGAS bearing K27-linked polyUb catalyzed by RNF185 in vitro according to Wang et al. 24 ( Figure S4 A) and then treated it[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Moreover, purified GST-OTUD3 protein, but not the GST alone, was able to interact with His-GRP78 under cell-free conditions (Fig.  xref ), suggesting a direct interaction between GRP78 and OTUD3."
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"18 , 23 After 293T shOTUD3#1 cells were transfected with FLAG-cGAS together with Myc-Ub WT or mutants, FLAG-cGAS bearing polyUb with various chain linkages was purified from cells and then treated wit[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In vitro pull-down assays with purified recombinant proteins showed that Myc-PLK1 directly bound GST-OTUD3, instead of GST control (Fig. xref )."
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