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PDLIM2 binds OTUD6B. 9 / 9
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"Ji and colleagues 219 identified a circRNA called Hsa‐circ‐0003764 (circPTPN12) from the circRNA database, discovering its role in suppressing hepatocellular carcinoma progression by interacting with the PDZ domain of PDLIM2, promoting P65 ubiquitination and the assembly of the PDLIM2/OTUD6B complex, thereby highlighting the ESRP1/circPTPN12/PDLIM2/NF‐κB axis as a potential therapeutic target."
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"Consistently, a Co-IP assay revealed the binding of OTUD6B to PDLIM2 (Fig.  xref A)."
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"Furthermore, circPTPN12 bolstered the assembly of the PDLIM2/OTUD6B complex by promoting the deubiquitination of PDLIM2."
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"To investigate the role of circPTPN12 in this context, we conducted immunoprecipitation experiments and observed reduced binding of PDLIM2 and OTUD6B upon circPTPN12 knockdown, whereas overexpression of circPTPN12 resulted in increased binding of PDLIM2 and OTUD6B. We discovered that circPTPN12 serves as a scaffold, facilitating the interaction between PDLIM2 and OTUD6B, promoting PDLIM2 deubiquitination, maintaining its expression, and exerting its anti-cancer function."
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"Consistently, a Co-IP assay revealed the binding of OTUD6B to PDLIM2 (Fig. 5A)."
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"RNA pull-down assays confirmed the association between PDLIM2, OTUD6B and circPTPN12 (Fig. 5C)."
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"Additionally, the Co-immunoprecipitation (Co-IP) assay showed a decrease in the binding between OTUD6B and PDLIM2 in circPTPN12 knockdown cells compared to control cells."
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"To investigate the role of circPTPN12 in this context, we conducted immunoprecipitation experiments and observed reduced binding of PDLIM2 and OTUD6B upon circPTPN12 knockdown, whereas overexpression of circPTPN12 resulted in increased binding of PDLIM2 and OTUD6B."
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"We discovered that circPTPN12 serves as a scaffold, facilitating the interaction between PDLIM2 and OTUD6B, promoting PDLIM2 deubiquitination, maintaining its expression, and exerting its anti-cancer function.The regulation of circRNA biogenesis is intricate."
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