IndraLab

Statements

USP4 deubiquitinates BRCA1. 6 / 6
| 6
reach
"USP4 physically interacts with and deubiquitinates BRCA1, thus saving BRCA1 from UPS-meditated ubiquitination and degradation."
38734703
reach
"USP4 deubiquitinates BRCA1."
38734703
reach
"Indeed, ectopic expression of USP4 WT, but not the C311A mutant, which is still capable of interacting with BRCA1, reduced both endogenous BRCA1 ubiquitylation (Fig. 3a, mostly polyubiquitinated BRCA1 species at high MW) and exogenous-overexpressed BRCA1 ubiquitylation in cells (Fig. 3b), suggesting that the DUB catalytic activity of USP4 is critical for USP4-dependent deubiquitylation of BRCA1."
38734703
reach
"Conversely, silencing USP4 expression increased endogenous BRCA1 ubiquitylation level in cells after MG132 treatment (Fig. 3c)."
38734703
reach
"As expected, USP4 WT efficiently deubiquitinated BRCA1."
38734703
reach
"We demonstrate that USP4 act as BRCA1 deubiquitinating enzyme to antagonize BRCA1 ubiquitination."
38734703