IndraLab

Statements

USP29 binds TWIST1. 14 / 14
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"As shown in Figure  xref , only the full‐length (FL) and M4 mutant (687‐922aa) region of USP29, but not other mutants, could interact with TWIST1."
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"We next confirmed the endogenous TWIST1‐USP29 interaction by co‐immunoprecipitation assay."
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"We first examined whether CDK1 regulated the interaction between USP29 with TWIST1."
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"All three sites are outside of the domain (687‐922aa) that mediates the interaction between USP29 and TWIST1."
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"The direct interaction of USP29 and TWIST1 prompted us to examine a potential role of USP29 in the regulation of TWIST1 stability and function."
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"As shown in Figure xref (Supporting Information), the treatment of RO‐3306 in MDA‐MB‐231 cells did not affect the USP29‐TWIST1 interaction."
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"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."
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"USP29 directly binds, deubiquitinates, and stabilizes TWIST1, which in turn promotes TWIST1‐driven tumor progression in TNBC."
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"The direct interaction of USP29 and TWIST1 prompted us to examine a potential role of USP29 in the regulation of TWIST1 stability and function."
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"In addition, purified GST‐USP29 but not GST pulled down His‐TWIST1 in vitro, indicating the direct interaction between USP29 and TWIST1 (Figure 1D)."
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"USP29 directly interacts with TWIST1, decreases its ubiquitination level through the DUB activity, thereby leading to the stabilization of TWIST1 (Figures  xref and  xref ; Figures xref and xref , Supporting Information)."
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"All three sites are outside of the domain (687‐922aa) that mediates the interaction between USP29 and TWIST1."
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"In addition, purified GST‐USP29 but not GST pulled down His‐TWIST1 in vitro, indicating the direct interaction between USP29 and TWIST1 (Figure  xref )."
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"Thus, we performed tandem affinity purification and mass spectrometry analysis in order to identify the potential regulators of the USP29‐TWIST1 axis."
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