IndraLab

Statements

USP13 is phosphorylated on T196. 11 / 11
| 5 6
rlimsp
"To further demonstrate the direct interaction between the MDC1 FHA domain and phosphorylated Thr 196 of USP13, T196 or p-T196 peptide was incubated with purified GST-MDC1 FHA domain in vitro."
28569838
rlimsp
"To further characterize the biological significance of USP13 phosphorylation, we stably expressed USP13 WT and T196A mutant in USP13-deficient cells."
28569838
sparser
"Recently, some scientists have reported that ATM is activated to phosphorylate USP13 at Thr196 site in response to DNA damage, stabilizing RAP80 to assemble BRCA1 to repair DSBs xref ."
29396516
sparser
"Aurora B and ataxia telangiectasia mutated (ATM) regulate the expression of USP13 via phosphorylation of USP13 at Ser114 and Thr196 to promote the interaction between them, respectively, while the CDC-like kinase 3 (CLK3)-Q607R mutation induces the phosphorylation of USP13 at Tyr708 and promotes its association with c-Myc ( xref )."
38192665
sparser
"Recently, researchers from Mayo Clinic uncover that ATM is activated in response to IR damage, and then phosphorylates USP13 at Thr196 site to stabilize RAP80, leading to the efficient recruitment of BRCA1 to the damage sites xref ."
29396516
sparser
"To further demonstrate the direct interaction between the MDC1 FHA domain and phosphorylated Thr 196 of USP13, T196 or p-T196 peptide was incubated with purified GST-MDC1 FHA domain in vitro ."
28569838
rlimsp
"Recently, researchers from Mayo Clinic uncover that ATM is activated in response to IR damage, and then phosphorylates USP13 at Thr196 site to stabilize RAP80, leading to the efficient recruitment of BRCA1 to the damage sites."
29396516
rlimsp
"To further confirm these results, we generated site-specific antibody against p-Thr196 and examined the USP13 phosphorylation following DNA damage."
28569838
rlimsp
"As shown in Supplementary Fig. 8c, T196 was phosphorylated after DNA damage, and the T196A mutation inhibited USP13 phosphorylation after DNA damage."
28569838
rlimsp
"As shown in Supplementary Fig. 8c, T196 was phosphorylated after DNA damage, and the T196A mutation inhibited USP13 phosphorylation after DNA damage. In addition, USP13 was phosphorylated on Thr196 following DNA damage in ATM-proficient cells but not ATM-deficient cells (Fig. 5c), suggesting ATM phosphorylates USP13 on T196 following DNA damage."
28569838
sparser
"Moreover, ATM-induced phosphorylation of the Thr196 residue in USP13 after DNA damage functions as an essential regulatory event, and plays a critical role in the resistance of cancer cells to chemotherapy by deubiquitinating RAP80, promoting recruitment of complexes, and eliciting a DDR ( xref ; xref ) ( xref )."
34177595