IndraLab

Statements

YOD1 binds GST. 5 / 5
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"BL21 competent cells were incubated with GST-YOD1 fastened Glutathione Sepharose."
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"In briefly, GST vector (pGEX-4T-1) and GST-YOD1 were transformed into the BL21 bacterial strain and incubated in Luria-Bertani broth (cat. no."
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"Meantime, recombinant GST-YOD1 and HIS-CDK1 proteins were purified, and the GST pulldown results further excluded the direct interaction between YOD1 and CDK1 ( Fig. xref )."
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"GST pull-down assay was performed essentially as described [ 37 ], except that a GST-YOD1 vector was used instead of a GST-USP7 vector."
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"Next, we performed the GST pull-down assay to check the direct interaction between YOD1 and α-synuclein, and found that GST-YOD1 precipitates α-synuclein ( Fig. 1 B)."
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