IndraLab

Statements

CLASP1 binds USP28. 12 / 12
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"To delineate the molecular mechanism by which USP28 regulates MAST1 protein, we first examined the interaction between USP28 and MAST1 under physiological conditions."
38498222
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"These findings confirm that USP28 interacts with MAST1 protein at both endogenous and exogenous levels."
38498222
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"We further used Duolink PLA assays to validate the interaction between USP28 and MAST1 under physiological conditions."
38498222
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"The in situ USP28-MAST1 interaction was observed as red signals (PLA dots) when USP28 and MAST1 were immunostained together, but not when they were stained with individual antibodies (Fig.  xref C)."
38498222
sparser
"On the basis of the above observations, we hypothesized that the interaction between USP28 and MAST1 might influence on MAST1 protein turnover."
38498222
reach
"We further used Duolink PLA assays to validate the interaction between USP28 and MAST1 under physiological conditions."
38498222
reach
"The interaction between USP28 and MAST1 was observed using a Duolink in situ proximity ligation assay (PLA) kit (Cat."
38498222
reach
"To delineate the molecular mechanism by which USP28 regulates MAST1 protein, we first examined the interaction between USP28 and MAST1 under physiological conditions."
38498222
reach
"On the basis of the above observations, we hypothesized that the interaction between USP28 and MAST1 might influence on MAST1 protein turnover."
38498222
sparser
"The high mRNA expression score of USP28 was proportional with MAST1 expression (Fig.  xref C; Supplementary Table S5) and showed a positive correlation between USP28-MAST1 exhibiting r value of 0.36 (Fig.  xref D)."
38498222
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"We envision that targeting the USP28-MAST1 axis along with cisplatin treatment might be an alternative therapeutic strategy to overcome cisplatin resistance in cancer patients."
38498222
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"Using a genome‐wide CRISPR–Cas9‐based screen, Karapurkar et al. identified USP28 as a candidate deubiquitinase (DUB) governing cisplatin resistance. xref Targeting the USP28–MAST1 axis alongside cisplatin therapy may offer an alternative strategy to overcome cisplatin resistance in cancer patients."
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