IndraLab

Statements

WWP2 binds USP9X. 9 / 9
3 | 6
biogrid
No evidence text available
25071155
sparser
"Preparation of the FAM-WWP2 working solution and MST pre-test."
33484499
sparser
"As we describe in the example outlined in Basic Protocol 4, we generate N-terminal fluorescein labeled protein FAM-WWP2 using 5/6-carboxyfluorescein NHS ester."
33484499
sparser
"The FAM-WWP2 is purified to be at 2 mg/ml concentration, with around 80% labeling efficiency."
33484499
sparser
"We use a working concentration of FAM-WWP2 at 50 nM in the MST binding assay."
33484499
sparser
"We prepare 16 serial ligand E2 concentrations by 1:2 dilution and measure the binding affinity to FAM-WWP2 in the MST binding assay."
33484499
biogrid
No evidence text available
33961781
sparser
"Here, we detail a protocol to label WWP2 at the N-terminus using Fluorescein NHS ester (5/6-carboxyfluorescein succinimidyl ester, FAM-NHS ester) adopting a stepwise labeling procedure (Basic Protocol 1 and 2), and then use the labeled FAM-WWP2 to measure its binding affinity to its requisite E2 (UbcH5c) enzyme by performing MST binding assays (Basic Protocol 3)."
33484499
biogrid
No evidence text available
31048545