IndraLab

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EIF3H binds CB. 4 / 4
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sparser
"These results demonstrate that CB interacts with eIF3H in mammalian cells."

sparser
"To investigate whether CB and eIF3H associate in a mammalian cell context, coimmunoprecipitation assays were carried out."

sparser
"Yeast-two-hybrid assay using the human CB lacking the SH3 domain (bait protein GAL4BD-CB SH3- ) showed that this truncated form of CB can also interact with eIF3H, indicating that this domain is not required to mediate the interaction with eIF3H (Figure xref )."

sparser
"Also, through YTH assay, we showed that the SH3 domain of CB is not required for interaction with eIF3H. Interaction of CB and eIF3H was further substantiated by coimmunoprecipitation of transiently expressed proteins in 293T cells."