IndraLab

Statements

USP9X activates fluorescein (lactone form). 4 / 4
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"Fluorescein labelled peptides were synthesized for VIP-82, ATSP-7041 and sMTIDE-02, by addition of FAM to their N-terminus via a beta-alanine linker, and then tested in the T22 p53 reporter assay to ensure that they retained comparable biological activity to their unlabeled analogs, respectively."
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"FAM labeled oligonucleotides were synthesized using fluorescein labeled phosphoramidite."
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"It is the product of biotin and carboxyl fluorescein (FAM) labels amplified by RAA, which applies antigen antibody binding to form "fluorescein antibody-double labeled nucleic acid amplification product-colloidal gold composite" at the detection line."
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"The absolute assembly rates were determined by measuring time‐resolved fluorescence anisotropy of 10‐mer RNA with a MeV genome 5′ sequence, labelled at the 3′ terminus with a fluorescein derivative (5′‐RNA ‐FAM; a longer RNA was used to allow the fluorescein to exit the RNA binding groove and to reduce the potential impact on assembly)."
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