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TP53 binds OTUD3. 20 / 20
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"The interaction between OTUD3 and p53 in BC cells was verified by a co-immunoprecipitation assay and GST pulldown experiments."
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"To determine whether the OTUD3-TP53 interaction was direct, we generated and purified recombinant Myc-OTUD3, Myc-D1 and Myc-D2."
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"OTUD3 interacts with p53."
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"Mechanistically, OTUD3 interacts directly with p53 through the amino-terminal OTU region."
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"The finding that OTUD3 potentially binds the N-terminus of p53 may suggest that both Mdm2 and OTUD3 compete for the same binding site in p53, possibly explaining the observed effects of OTUD3 overexpression and knockdown on p53 ubiquitination and p53 levels in cells."
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"In summary, we found that OTUD3 may be a potential therapeutic target for restoring p53 function in breast cancer cells and suggest that the OTUD3-p53 signaling axis may play a critical role in tumor suppression."
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"To determine the region where p53 binds OTUD3, we also constructed truncated p53 mutants (Fig. xref f) and performed GST assays."
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"Using the D1 and D2 constructs in co-immunoprecipitation experiments, we revealed that the OTU domain-containing region D1 (1–183) is critical for the interaction between OTUD3 and p53 (Fig. xref d)."
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"The finding that OTUD3 potentially binds the N-terminus of p53 may suggest that both Mdm2 and OTUD3 compete for the same binding site in p53, possibly explaining the observed effects of OTUD3 overexpression and knockdown on p53 ubiquitination and p53 levels in cells."
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"The interaction between OTUD3 and p53 in BC cells was verified by a co-immunoprecipitation assay and GST pulldown experiments."
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"Mechanistically, OTUD3 interacts directly with p53 through the amino-terminal OTU region."
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"Using the D1 and D2 constructs in co-immunoprecipitation experiments, we revealed that the OTU domain-containing region D1 (1–183) is critical for the interaction between OTUD3 and p53 (Fig. 4d)."
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"The OTUD3-p53 interaction may be involved in the formation of BC."
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"Interestingly, Myc-D1 cultured from MCF7 cells was specifically bound by the purified GST-TP53 protein but not GST alone (Fig. xref e), further illustrating the direct interaction between OTUD3 and p53."
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"The results indicated that OTUD3 bound the following two mutants of p53: T2 (1–113)/(290–393) and T3 (1–113)/(236–393) (Fig. xref g, h)."
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"OTUD3 can directly interact with and stabilize p53 through deubiquitination in BC cells."
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"Interestingly, Myc-D1 cultured from MCF7 cells was specifically bound by the purified GST-TP53 protein but not GST alone (Fig. 4e), further illustrating the direct interaction between OTUD3 and p53."
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"To determine the region where p53 binds OTUD3, we also constructed truncated p53 mutants (Fig. 4f) and performed GST assays."
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"These results suggest that a direct interaction exists between OTUD3 and p53."
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"Co-immunoprecipitation (CO-IP) assays were conducted in MCF7 cells and DU4475 cells to examine whether OTUD3 physically interacts with p53."
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