IndraLab

Statements

USP20 binds YAP1. 12 / 12
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"USP20 directly interacts with YAP1 in a DUB-dependent protease-related regulatory mechanism."
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"To investigate the regulatory mechanism of USP20-mediated upregulation of YAP1 expression, reciprocal co-immunoprecipitation was performed in T24 cells to identify a potential physical interaction between USP20 and YAP1 ( xref A)."
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"The results of a GST pull-down assay confirmed that USP20 and YAP1 directly bind to each other ( xref B)."
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"We monitored the direct interaction between USP20 and YAP1 using immunofluorescence ( xref D) and Duolink proximity ligation assays ( xref F)."
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"These results indicated that USP20 directly interacts with YAP1 and increases its stability by preventing ubiquitin-proteasome pathway activity."
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"To investigate the regulatory mechanism of USP20-mediated upregulation of YAP1 expression, reciprocal co-immunoprecipitation was performed in T24 cells to identify a potential physical interaction between USP20 and YAP1 (Fig. 4A)."
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"Thus, we performed further experiments to determine the details of the interaction between USP20 and YAP1."
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sparser
"Co-IP and GST pull-down assays indicated that USP20 directly interacted with YAP1 ( xref )."
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"Thus, we performed further experiments to determine the details of the interaction between USP20 and YAP1."
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"For interaction analysis, deleted constructs were cloned and analyzed, which revealed that the WW domain of YAP1 is essential for its interaction with USP20 ( xref A) and that the UCH domain is required for the direct interaction of USP20 with YAP1 ( xref B)."
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"USP20 directly interacts with YAP1 and inhibits its K48‐linked polyubiquitination, thereby enhancing the expression of YAP1 and its downstream target genes. xref In T‐cell acute lymphoblastic leukaemia (T‐ALL), USP20 has been identified as a super‐enhancer‐regulated driver gene that promotes tumour cell survival by deubiquitinating and stabilizing HIF1A. The USP20 inhibitor GSK2643943A exhibits significant anti‐tumour activity. xref Through multiple mechanisms, USP20 maintains the activity of oncogenic signalling pathways and participates in tumour cell hypoxia adaptation, proliferation and invasion."
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"Analysis of the underlying mechanisms revealed that USP20 directly interacted with the YAP1 protein and promoted its stability through inhibition of K48-linked poly-ubiquitination."
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