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OTUB1 binds ypkA. 5 / 5
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"To test the interaction between otubain 1 and YpkA, HEK293 cells were co-transfected with HA-tagged variants of wild type or mutant (D88A, C91S) otubain 1 and FLAG-epitope tagged YpkA K269A, a kinase [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Interestingly, like other YpkA substrates, pulldown assays showed that OTUB1 also interacts with YpkA ( xref ; xref )."
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"It is likely that this phosphorylation modulates bacterial entry and uptake within the cell due to actin dependence and YpkA/OTUB1 complexing with the small GTPase RhoA (Edelmann et al., 2010)."
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"YpkA was immunoprecipitated using anti-FLAG M2 affinity resin and anti-HA immunoblot analysis confirmed that otubain 1 associates with YpkA ( Fig. 4 B)."
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"YpkA was immunoprecipitated using anti-FLAG M2 affinity resin and anti-HA immunoblot analysis confirmed that otubain 1 associates with YpkA ( Fig. 4 B)."
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