IndraLab

"H2A-H2B promotes Sad1 phase separation."

"Together, these data demonstrate that H2A-H2B promotes Sad1 phase separation to enrich Sad1-interaction partners, such as HDACs, in the same condensates.Collectively, we propose the following model for Sad1’s role in heterochromatin positioning and formation: H2A-H2B interacts with Sad1 and facilitates the otherwise-dispersed Sad1 clustering into condensates, forming discrete Sad1 puncta distributed on the NE as observed in Fig. 3; liquid droplet-like condensates formed by Sad1-H2A-H2B help recruit heterochromatin factors, such as Clr3 and Sir2, to the condensates, which promotes spatial organization and heterochromatin silencing at the nuclear envelope (Fig. 7c)."

"H2A-H2B enhances the phase separation ability of Sad1, which in turn facilitates the recruitment of heterochromatin factors."

"We hypothesized that H2A-H2B might promote Sad1 phase separation to recruit heterochromatin factors to the same condensates to facilitate heterochromatin organization."