IndraLab

"Besides, Co-IP assay showed that the interaction between SRPX2 and OTUB1 was strengthened with circSEC24B transfection, but not circSEC24B-MUT transfection (Fig. xref )."

"CircSEC24B served as a scaffold to enhance the binding of SRPX2 proteins with OTUB1."

"Considering that both circSEC24B and OTUB1 can regulate the expression of SRPX2, and circSEC24B and OTUB1 bind to each other, we hypothesized that circSEC24B functions as a scaffold to enhance the binding of SRPX2 with OTUB1."

"To further explore whether the interaction between OTUB1 and SRPX2 was affected by the acetylation level of SRPX2."

"As expected, the co-IP experiment results indicated that the binding of OTUB1 to SRPX2 was weakened in a dose-dependent manner after the treatment of NAM."

"Interactions between circSEC24B, OTUB1, and SRPX2 were analyzed through immunofluorescence, RNA-pulldown, and RIP assays."

"Interactions between circSEC24B, OTUB1, and SRPX2 were analyzed through immunofluorescence, RNA-pulldown, and RIP assays."

"Specifically, circSEC24B acted as a scaffold, facilitating the binding of OTUB1 to SRPX2 and thereby enhancing its protein stability."

"In addition, the mutual co-immunoprecipitation assay indicated that SRPX2 and OTUB1 bound to each other in the LoVo and HCT116 cell lines (Fig. xref )."

"In vitro binding assays revealed that O-3 domain (85-271 amino acids) of the MYC-tagged OTUB1 protein binds to SRPX2 (Fig. xref )."

"Specifically, circSEC24B acted as a scaffold, facilitating the binding of OTUB1 to SRPX2 and thereby enhancing its protein stability."

"ncRNAs (e.g., circular RNAs [circRNAs] and long non-coding RNAs [lncRNAs]) can act as molecular scaffolds, conferring spatial and substrate specificity to OTUB1 through the formation of ternary complexes. xref , xref For example, hsa_circ_0005185 simultaneously binds both OTUB1 and its substrate RAB8A, creating a spatially constrained catalytic environment to promote RAB8A deubiquitination and stabilization, thereby inhibiting prostate cancer progression. xref , xref Similarly, circSEC24B facilitates the interaction between OTUB1 and SRPX2 via its scaffolding function, enhancing SRPX2 stability to activate autophagy and promote colorectal cancer cell proliferation and chemoresistance. xref LncRNAs expand this scaffolding paradigm."