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USP6 binds GOLPH3. 10 / 10
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"Through mechanical binding, circCYFIP2 enhances the interaction between GOLPH3 and USP6, inducing DDP resistance both in vitro and in vivo.To sum up, USP6 functions as a deubiquitinase that targets and increases the stability of the GOLPH3 protein in COAD."
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"Notably, circCFYIP2 interacted with both GOLPH3 and USP6 protein, and facilitated GOLPH3 deubiquitination through an intensified binding of USP6 to GOLPH3, which resulted in platinum resistance in COA[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Therefore, CoIP assay was performed to confirm the direct interaction between USP6 and GOLPH3."
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"As shown in Fig. 2 A, Flag-tagged GOLPH3 was able to pulldown HA-tagged USP6 and vice-versa in HEK293T cells, indicating that USP6 interacts with GOLPH3."
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"Interestingly, after GOLPH3 was IP from HEK293T cells with circCYFIP2 silence, the following USP6 level was downregulated ( Fig. 10 D), which revealed that circCYFIP2 knockdown significantly hindered [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38735445
sparser
"Through mechanical binding, circCYFIP2 enhances the interaction between GOLPH3 and USP6, inducing DDP resistance both in vitro and in vivo."
40348771
sparser
"CircCFYIP2 was found to directly interact with USP6 and GOLPH3 protein, thereby strengthening the deubiquitination process of GOLPH3 mediated by USP6, which ultimately enhanced GOLPH3 stability and pr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38735445
sparser
"Notably, circCFYIP2 interacted with both GOLPH3 and USP6 protein, and facilitated GOLPH3 deubiquitination through an intensified binding of USP6 to GOLPH3, which resulted in platinum resistance in COA[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38735445
reach
"Therefore, CoIP assay was performed to confirm the direct interaction between USP6 and GOLPH3."
38735445
reach
"Interestingly, after GOLPH3 was IP from HEK293T cells with circCYFIP2 silence, the following USP6 level was downregulated ( Fig. 10 D), which revealed that circCYFIP2 knockdown significantly hindered [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38735445