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"Analyzing the UBA domain from Cezanne or Cezanne2 binding to K63-di-Ub in vitro using isothermal titration calorimetry (ITC) showed that both of the UBA domains bind to K63-di-Ub, with a binding affinity (K = 20 ± 2 and 20 ± 5 μM), approximately threefold lower than that of the UIMs of Rap80 (K = 6.4 ± 0.2 μM) (Fig. 1C)."