IndraLab

Statements

CALM1 binds CARF. 8 / 8
| 8
sparser
"We found that cA 6 occupies, but does not entirely fit, the same pocket of the Nh Cam1-CARF dimer that binds cA 4 (Extended Data Fig. xref )."
38200316
sparser
"Notably, the apo Nh Cam1-CARF dimer crystal structure aligned to the CARF domain of the model with a root mean-squared deviation of 0.44 Å."
38200316
sparser
"Structural and biochemical analyses reveal that the CARF domains of a Cam1 dimer bind cyclic tetra-adenylate second messengers."
38200316
sparser
"We next grew crystals and solved the structure Nh Cam1-CARF (residues 42–206) in the absence and presence of bound cA 4 ."
38200316
sparser
"The 2.2 Å-resolution X-ray structure without the ligand showed that Nh Cam1-CARF adopts a domain-swapped dimeric topology, in which the β7 strand of one monomer completes the folding topology of a second monomer by pairing with its β6 strand (Fig. xref and Extended Data Fig. xref )."
38200316
sparser
"By contrast, the 2.1 Å X-ray structure of Nh Cam1-CARF bound to cA 4 shows that it adopts a dimeric fold with no domain swap between the monomers (Fig. xref )."
38200316
sparser
"Loop L2 adopts different conformations in each monomer of the Nh Cam1-CARF dimer in the complex, with the N and C termini being in close proximity in one monomer but far apart in the other monomer, owing to crystal packing forces."
38200316
sparser
"Finally, despite not observing binding of cA 6 in our ITC experiments (Extended Data Fig. xref ), we were able to crystallize and solve the 1.9 Å X-ray structure of Nh Cam1-CARF (residues 42–206) bound to this ligand."
38200316