IndraLab

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AR binds USP22. 9 / 11
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"This result together with the observed interaction between the AR and both ATXN7L3 and USP22 suggested that these proteins are recruited together to the promoters of AR dependent genes upon ligand ind[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Cotransfection in HEK293T cells and coimmunoprecipitation experiments revealed that AR interacted with ATXN7L3 in a ligand-dependent manner, similarly to GCN5, while interaction between AR and USP22 w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Subsequent immunoprecipitation also confirmed the association between AR and USP22 in vitro, which is consistent with the result of previous studies (Fig. 3I)."
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"As AR and USP22 interacted in vivo, we next tested whether AR could be a substrate of the TFTC/STAGA deubiquitination activity."
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"No significant association of AR and USP22 with chromatin at an intergenic region could be detected, and the amount of histone H3 at KLK2 promoter remained constant, further demonstrating specificit[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In addition, our immunofluorescence analysis showed that Importin-7 binds to AR or USP22 in both the nucleus and cytoplasm (Fig. 4C–E), suggesting that Importin-7 may regulate the nuclear translocation of AR and USP22.Next, we conducted a more in-depth study of the Importin-7–AR–USP22 complex by the treatment of AR inhibitor (enzalutamide) or AR ligands, including DHT and synthetic androgen R1881."
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"Here, to further investigate whether the arrest of the nuclear translocation of AR–USP22 complex triggered by the knockdown of Importin-7 could enhance the sensitivity of BC to enzalutamide.We first confirmed that the knockdown of Importin-7 could enhance the growth inhibition of cancer cells by enzalutamide using CCK8 assay (Fig. 6A, B) and EdU staining assay (Fig. 6C, D)."
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"Interestingly, we found that USP22, one of the maintainers of AR [22–24], whose nuclear translocation is also regulated by Importin-7, although knockdown of Importin-7 may not affect the formation of the AR–USP22 complex.In addition, we also observed that AR expression levels were upregulated after DHT and R1881 stimulation, and Importin-7-bound AR was also up-regulated to an almost equal extent, indicating that the level of AR carried by Importin-7 was not saturated."
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"Cotransfection in HEK293T cells and coimmunoprecipitation experiments revealed that AR interacted with ATXN7L3 in a ligand dependent manner, similarly to GCN5, while interaction between AR and USP22 w[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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