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Delta-actitoxin-Avd1a binds USP2. 409 / 409
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"Also, the outcomes of transwell assays exhibited that USP2-AS1 silence successfully inhibited ovarian cancer cell migration to a great extent, which was abolished after inhibiting miR-520d-3p; and KIAA1522 depletion further reversed above phenomenon induced by miR-520d-3p inhibition ( xref )."
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"Foetal macrosomia is also induced by a placental increase in certain genes in mothers without GDM, such as placental expression of PPAR alpha and gamma , which are considered transcriptional regulators with key functions in lipid metabolism and miR-27b . xref On the other hand, the effects of long non-coding RNAs (lncRNA) in non-diabetic foetal macrosomia have also been studied, suggesting that the lncRNA USP2-AS1 promotes placental development by affecting the proliferative activity of placental cells. xref Moreover, another study indicated that lncRNA-SNX17 overexpression reduces the expression of miR-517a , which could play an important role in the regulation of birth weight, and increases the expression of IGF-1 in the human trophoblast cell line HTR-8/SVneo, thus enhancing the proliferation of HTR-8/SVneo. xref It also reported that lncRNA-SNX17 could promote trophoblast proliferation through the miR-517a/IGF-1 pathway and could play a role in diabetic macrosomia placentation. xref However, further studies are required to elucidate the underlying mechanisms."
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"HOTAIR could be regarded as a potential indicator of cell cycle dysregulation in lung cancer. xref MEG3 was down-regulated in ovarian cancer cells and elicited tumor inhibitor role in this disease. xref Besides, lncRNAs have also been reported to affect tumor growth, metastasis, and drug resistance by controlling target gene expression and pathway activation. xref , xref For example, TUC338 accelerated the development of lung cancer via targeting MAPK pathway. xref CCAT1 affected breast cancer development via miR-148b. xref Unfortunately, the role and mechanism of ubiquitin specific peptidase 2 antisense RNA 1 (USP2-AS1) in ovarian cancer are still not well understood."
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"Regarding previously reported lncRNAs HHIP-AS1 and LOXL1-AS1 , DEA showed differential expression in SHH group as expected, while tumor suppressor lncRNA NKX2-2AS was upregulated in this group. xref LINC-NED125 , CRNDE , and OTX2-AS1 , which affect tumor progression in MB, showed differential expression patterns between groups. xref , xref , xref Additionally, CCAT1 and USP2-AS1 were found upregulated, while MIR100HG was downregulated in Gr3 MBs, consistent with prior findings. xref The expression profiles observed in our study not only confirmed the relevance of several previously characterized oncogenic and tumor-suppressive lncRNAs but also revealed novel group-specific lncRNAs which could be key molecular group biomarkers of MB."
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"For example, LncRNA USP2-AS1 could promote colon cancer development through Hippo/YAP1 pathway. xref SNHG17 regulated the expression of CBX3 by binding to miR-375, thus promoting the progression of colon cancer. xref LINC01578 promoted colon cancer metastasis by forming positive feedback with the NF-κB/YY1 axis. xref In this study, we found that ILF3-AS1 expression was significantly higher in COAD samples and cell lines than in the control group."
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"The current data have demonstrably proved that a majority of lncRNAs impact the development of ovarian cancer by mediating cell proliferation and apoptosis, even migration and invasion. xref , xref For example, TP73-AS1 knockdown hampered ovarian cancer cell growth by modulating cell apoptosis, and also effectively elevated the survival time of patients with this disease. xref MALAT1 stimulated epithelial ovarian cancer cell viability as well as cell migration and invasion. xref Moreover, DUXAP10 was indicated as a potential therapeutic biomarker for ovarian cancer. xref In the present work, we discovered from the GEPIA database that USP2-AS1 was highly-expressed in ovarian cancer tissues."
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"Through RT-qPCR and western blot analyses, we found that expressions of osteogenesis-associated markers, including RUNX2, ALP, OSX and OCN were gradually increased after osteogenic induction, while USP2-AS1 depletion evidently reduced the expressions of the indicated markers (Fig. S2E-F & xref )."
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"The suppression of MYC induces cellular senescence in different types of cancer cells, including osteosarcoma and hepatocellular carcinoma. xref MYC enables cancer cells to resist senescence by being dependent on cell cycle protein-dependent kinase 2 (CDK2). xref MYC inhibits senescence by activating long noncoding RNA (lncRNA) USP2-AS1 to stabilize E2F1 mRNA. xref "
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"The experimental results in the current study firstly verified the promoting effects of USP2-AS1 on the osteogenic differentiation of HBMSCs, which was evidenced by the results that USP2-AS1 knockdown led to the decreased ALP activity, reduced formation of mineralized bone matrix and lessened expression levels of osteogenesis-associated markers (RUNX2, ALP, OSX and OCN)."
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"The expression of CFAP43 were regulated by hsa-miR-3120-3p which may be sponged by hsa_circ_0007161, hsa_circ_0000996, hsa_circ_0069606, hsa_circ_0027707, hsa_circ_0003677, hsa_circ_0028190, hsa_circ_0052575, hsa_circ_0001513, LINC00504, USP2-AS1, LINC00535, LINC00271, AP000708.1, or DNAJC27-AS1."
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"It was reported that some lncRNAs, such as ARHGEF7-AS2, lnc-HLX-1, lnc-EXPH5-2, lnc-CH25H-2, and lnc-TDRP-3, demonstrate differential expression in these two groups, and that other lncRNAs were subgroup-specific: lnc-CCL2-2 in the WNT subgroup, lnc-ABCE1-5 in the SHH subgroup, USP2-AS1 in group 3, and lnc-TBC1D16-3 in group 4 [ xref ]."
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"We then performed dual-luciferase reporter assays, as illustrated in xref D. The USP2-AS1 promoter reporters pHREs1+2, and especially the pHREs2 region, could be significantly responsive to hypoxia in FaDu cells ( xref D); we also noticed that pHREs1 responded moderately to hypoxia, and it seemed that HIF1α was mainly bound to the pHREs2 region."
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"To validate the sgGSEA results, we determined the expression of cell proliferation-, death-, and glycosis-associated genes in these subcutaneous tumors and found that the knockdown of USP2-AS1 inhibited the expression of cell-cycle-progression- and glycosis-associated genes ( xref H)."
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"Most of the potential binding proteins of USP2-AS1 are listed in xref B. Next, we analyzed the clinical significance of these proteins in the TCGA-HNSCC dataset and found that DCAF13 (DDB1- and CUL4-associated factor 13), a substrate receptor for the CUL4–DDB1 E3 ubiquitin–protein ligase complex [ xref ], was also highly expressed ( xref C) and positively correlated with USP2-AS1 ( xref D): a higher level of DCAF13 predicted poorer prognosis ( xref E) in HNSCC patients."
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"It has been reported that some lncRNAs, such as ARHGEF7-AS2 , lnc-HLX-1 , lnc-EXPH5–2 , lnc-CH25H-2 and lnc-TDRP-3 , demonstrate differential expression in these two groups, and that other lncRNAs were subgroup-specific: lnc-CCL2–2 in the WNT subgroup, lnc-ABCE1–5 in the SHH subgroup, USP2-AS1 in group 3 and lnc-TBC1D16–3 in group 4 ( xref )."
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"This effect is further amplified by the long non-coding RNA USP2-AS1, which enhances transcriptional activation of the USP2 gene by facilitating interactions between KDM3A and ETS1 at the promoter region, thereby reinforcing β-catenin stability and promoting human bone marrow mesenchymal stem cell (HBMSC) differentiation into osteoblasts [ xref , xref , xref ]."
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"Notably, the expression of lncRNA USP2-AS1 is significantly upregulated in hypoxic microenvironments, activating downstream hypoxic response pathways by enhancing the interaction between the RNA-binding protein YBX1 and HIF1α mRNA, thereby stabilizing HIF1α mRNA and increasing its protein levels."
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"Importantly, the knockdown of USP2-AS1 markedly improved the therapeutic efficacy of the tyrosine kinase inhibitor lenvatinib and inhibited tumor growth in an in vivo model, indicating that targeting USP2-AS1 may reverse therapeutic resistance in hepatocellular carcinoma cells by disrupting HIF1α signaling ( xref )."
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"The following 29 lncRNAs were found to be associated with prognosis: AL009178.3, AC034199.1, AC017083.1, AC087588.1, AL354892.2, AC010336.7, AC125437.1, AC091153.3, AP003352.1, SCAT2, USP2-AS1, CTBP1-DT, AC016737.1, ALMS1-IT1, LINC01356, AC107032.2, AL355 SBNO1-AS1, AL024508.1 b, AC022784.6, AC008610.1, AC011298.1, AC145423.1, AC005914.1, and AC011298.1 ( xref )."
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"The study found that an 11-lncRNA signature ( MIR100HG , lnc-CFAP100-4 , ENSG00000279542 , lnc-ABCE1-5 , USP2-AS1 , lnc-RPL124 , OTX2-AS1 , lnc-TB1D16-3 , ENSG00000230393 , ENSG00000260249 , and lnc-CCL2 ) could accurately classify MB subgroups with an average < 7% class error rate."
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"By analyzing the positive cell rate of USP2-AS1 in human liver cancer tissue microarrays and determining the cutoff value of USP2-AS1 by X-Tile software, we observed that patients with low expression of USP2-AS1 had better prognosis while patients with high expression of USP2-AS1 had shorter OS (Overall Survival) and DFS (Disease-Free Survival) time ( xref )."
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"Our previous study showed that USP2-AS1 increased the protein levels of HIF1α in an anoxic environment, RT-qPCR also showed that the expression of target genes downstream of HIF1α was also significantly down-regulated in USP2-AS1 knockdown Huh7 cell line ( xref ), suggesting that USP2-AS1 knockdown may enhance the efficacy of lenvatinib."
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"Importantly, compared with control groups, the tumors’ weight and volume were the smallest in the group treated with lenvatinib and USP2-AS1 knockdown ( xref ), indicating that USP2-AS1 knockdown can enhance the therapeutic effect of lenvatinib in HCC in our mice tumor xenograft model."
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"Based on the multivariate Cox analysis, a PS formula was established using the following equation: PS = (-0.6536 × Exp SLC25A30-AS1) + (-0.5201 × Exp HPN-AS1) + (0.4276 × Exp LINC00607) + (0.6304 × Exp USP2-AS1) + (0.6317 × Exp HCG20) + (0.8265 × Exp LINC00638) + (1.1926 × Exp MKLN1-AS) + (3.0642 × Exp LINC00652)."
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"HPN-AS1 could be utilized in the diagnosis of prostate cancer ( xref ); LINC00607 facilitates the proliferation and invasion of osteosarcoma ( xref ); USP2-AS1 promotes the progression of colon cancer and ovarian cancer ( xref , xref ); and LINC00638 and LINC00652 have been reported in nontumor diseases ( xref – xref ), while no study has revealed the function of the remaining lncRNAs."
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"The hypoxia-associated lncRNA USP2-AS1 knockdown significantly inhibited the translation of HIF1α protein and the transcriptional activation of its downstream target gene, such as VEGF, and increased the inhibitory effect of lenvatinib on the growth of HCC mice xenograft tumors."
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"Lu et al. [ xref ] identified 892 differentially expressed lncRNAs (763 upregulated and 129 downregulated) based on microarray analysis of lncRNAs in 48 NDFMS and 48 control placentas, which were further validated by selecting lncRNA USP2-AS1 as a candidate lncRNA for subsequent experiments, and the downregulation of lncRNA USP2-AS1 was found to possibly participate in NDFMS development by promoting trophoblast cell viability."
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"Knockdown of USP2-AS1 can promote phosphorylation and ubiquitin-mediated degradation of Yes-associated protein 1 (YAP1) to inhibit the activation of the Hippo/YAP1 signaling pathway, which in turn inhibits the proliferative, migratory and invasive abilities of tumor cells and reduces the tumorigenicity and distant metastatic ability of cancer cells in mice ( xref )."
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"Previous studies have reported that lncRNAs may potentially participate in the pathogenesis of placental development ( xref – xref ); however, the specific biological effects of lncRNAs remain largely unknown, particularly concerning the regulatory role of ubiquitin-specific peptidase 2 antisense RNA 1 ( USP2-AS1 ) in NDFMS."