IndraLab

Statements

KRT1 binds OTUD4. 10 / 10
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"Overexpression of HA-OTUD4 interacted with FLAG-K-RTA in HEK293T cells, and endogenous OTUD4 also formed a complex with K-RTA when we induced KSHV lytic reactivation in both SLK."
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"These results confirmed that K-RTA interacts with OTUD4."
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"Interestingly, A C45A mutant that abrogates OTUD4 DUB activity [ xref , xref ] still interacted comparably with K-RTA, indicating that the DUB activity of OTUD4 is dispensable for K-RTA binding ( xref )."
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"Next, we mapped the regions that mediate the interaction between K-RTA and OTUD4."
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"Together, these data indicate that K-RTA interacts with OTUD4 and the C-terminal FRD motif of K-RTA and the N-terminal 1-425aa of OTUD4 mediate the interaction."
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"KSHV K-RTA interacts with OTUD4."
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"These data indicate that OTUD4 associates with K-RTA via the C-terminal FRD motif of K-RTA to facilitate KSHV lytic reactivation."
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"Since K-RTA plays a crucial role in KSHV reactivation and OTUD4 interacts with K-RTA, we hypothesized that OTUD4 may modulate K-RTA protein level to promote lytic reactivation."
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"Next, we mapped the regions that mediate the interaction between K-RTA and OTUD4."
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sparser
"In summary, we report that OTUD4 interacts with K-RTA and enhances K-RTA stability by promoting K-RTA deubiquitination."
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