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EN523 binds OTUB1. 3 / 3
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"In addition, they confirmed that EN523 covalently bound OTUB1 in live cells."

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"We next performed liquid chromatography-tandem mass spectrometry analysis (LC-MS/MS) of tryptic peptides from EN523 bound to OTUB1 and showed that EN523 selectively targets C23, with no detectable modification of the catalytic C91 (Figure 2d)."

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"We next used NMR analysis to further characterize EN523 binding to OTUB1."