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"Mass spectrometry analysis of Flag-OTUB2 isolated from MDA-MB-231 cells revealed possible SUMOylation at Lys233, which is just before the stop codon ( Figure 4 A; Table S5 )."

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"Indeed, co-expression of Flag-OTUB2 and Myc-SUMO3 resulted in OTUB2 poly-SUMoylation, which could be strongly enhanced by co-expression of PIAS3 ( Figure 4 B) and decreased by SENP1 ( Figure 4 C)."